Rapid detection of free radicals using spin trapping and MALDI-TOF mass spectrometry

被引：6

作者：

Podmore, Ian ^{[1
]}

Cunliffe, Laurie ^{[1
]}

Heshmati, Mayha ^{[1
]}

机构：

[1] Univ Salford, Biomed Sci Res Inst, Sch Environm & Life Sci, Salford M5 4WT, Lancs, England

来源：

JOURNAL OF CHEMICAL RESEARCH | 2013年 / 01期

关键词：

spin trapping; MALDI-TOF; alpha-[4-pyridyl N-oxide]-N-tert-butyl nitrone (POBN); free radicals; Fenton reaction;

D O I：

10.3184/174751912X13548917858338

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study we demonstrate that matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry may be used as a simple and fast alternative to current methods for detecting spin-trapped free radicals.

引用

页码：45 / 47

页数：3

共 9 条

[1] Mass spectrometry-based proteomics [J].

Aebersold, R ;

Mann, M .

NATURE, 2003, 422 (6928) :198-207

[2] Mechanisms of free radical-induced damage to DNA [J].

Dizdaroglu, Miral ;

Jaruga, Pawel .

FREE RADICAL RESEARCH, 2012, 46 (04) :382-419

[3] A binary matrix for background suppression in MALDI-MS of small molecules [J].

Guo, Zhong ;

Lin He .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 387 (05) :1939-1944

[4] SPIN TRAPPING [J].

JANZEN, EG .

ACCOUNTS OF CHEMICAL RESEARCH, 1971, 4 (01) :31-&

[5] DECAY AND FATE OF THE HYDROXYL RADICAL ADDUCT OF ALPHA-PHENYL-N-TERT-BUTYLNITRONE IN AQUEOUS-MEDIA [J].

KOTAKE, Y ;

JANZEN, EG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (25) :9503-9506

[6] Indirect detection of hydroxyl radicals using spin trapping and gas chromatography-mass spectrometry [J].

Mistry, Pritesh ;

Najim, Nigar ;

Purdie, Ashley ;

Podmore, Ian .

JOURNAL OF CHEMICAL RESEARCH, 2008, (07) :395-397

[7] A novel protocal to identify and quantify all spin trapped free radicals from in vitro/in vivo interaction of HO and DMDO: LC/ESR, LC/MS, and dual spin trapping combinations [J].

Qian, SY ;

Kadiiska, MB ;

Guo, QO ;

Mason, RP .

FREE RADICAL BIOLOGY AND MEDICINE, 2005, 38 (01) :125-135

[8] Acid-base-driven matrix-assisted mass spectrometry for targeted metabolomics [J].

Shroff, Rohit ;

Rulisek, Lubomir ;

Doubsky, Jan ;

Svatos, Ales .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (25) :10092-10096

[9] Desorption-ionization mass spectrometry on porous silicon [J].

Wei, J ;

Buriak, JM ;

Siuzdak, G .

NATURE, 1999, 399 (6733) :243-246

← 1 →