Anthocyanin Accumulation and Transcriptional Regulation of Anthocyanin Biosynthesis in Purple Pepper

被引：71

作者：

Tang, Boyan ^{[1
,2
]}

Li, Ling ^{[1
,2
]}

Hu, Zongli ^{[1
,2
]}

Chen, Yanan ^{[1
,2
]}

Tan, Tingting ^{[1
,2
]}

Jia, Yanhua ^{[1
,2
]}

Xie, Qiaoli ^{[1
,2
]}

Chen, Guoping ^{[1
,2
]}

机构：

[1] Chongqing Univ, Key Lab Bioengn Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China

[2] Chongqing Univ, Bioengn Coll, Chongqing 400044, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2020年 / 68卷 / 43期

基金：

中国国家自然科学基金;

关键词：

purple pepper; anthocyanin; HPLC-LC-MS; RNA-Seq; Y2H; BiFC; FLAVONOID BIOSYNTHESIS; PROVIDES INSIGHTS; ARABIDOPSIS; EGGPLANT; PROTEIN; GENES; PETUNIA; PATHWAY; COMPLEX; HEALTH;

D O I：

10.1021/acs.jafc.0c02460

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Pepper (Capsicum annuum) is among the important horticultural crops with economic value, and more and more colorful varieties have been marketed. The purple pepper is becoming increasingly popular on the consumer market because of its anthocyanin richness. Here, two cyanidin-based anthocyanins were separated and identified from peels of purple cultivars by HPLC -LC -MS. To study the molecular mechanism of anthocyanin accumulation, the differential expression of genes related to anthocyanin biosynthesis was examined by qRT-PCR and RNA-Seq in peel from green and purple cultivars. These results show that CaANT1, CaANT2, CaAN1, and CaTTG1 are involved in anthocyanin accumulation of pepper. Further investigation suggested that CaANT1, CaANT2, CaAN1, and CaTTG1 can activate anthocyanin accumulation via forming a new MMBW transcription complex.

引用

页码：12152 / 12163

页数：12

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