L-CYSTEINE PROMOTES THE PROLIFERATION AND DIFFERENTIATION OF NEURAL STEM CELLS VIA THE CBS/H2S PATHWAY

被引：62

作者：

Wang, Z. ^{[1
,2
]}

Liu, D. -X. ^{[1
]}

Wang, F. -W. ^{[1
]}

Zhang, Q. ^{[1
]}

Du, Z. -X. ^{[1
]}

Zhan, J. -M. ^{[1
]}

Yuan, Q. -H. ^{[1
]}

Ling, E. -A. ^{[3
]}

Hao, A. -J. ^{[1
]}

机构：

[1] Shandong Univ, Minist Educ Expt Teratol, Shandong Prov Key Lab Mental Disorders, Key Lab,Dept Histol & Embryol,Sch Med, Jinan 250012, Shandong, Peoples R China

[2] Shandong Univ, Inst Physiol, Sch Med, Jinan 250012, Shandong, Peoples R China

[3] Natl Univ Singapore, Dept Anat, Yong Loo Lin Sch Med, Singapore 117597, Singapore

来源：

NEUROSCIENCE | 2013年 / 237卷

基金：

中国国家自然科学基金;

关键词：

(L)-cysteine; neural stem cells; proliferation; differentiation; CYSTATHIONINE BETA-SYNTHASE; HYDROGEN-SULFIDE; NG108-15; CELLS; INVOLVEMENT; EXPRESSION; INHIBITOR; KINASE; DAMAGE;

D O I：

10.1016/j.neuroscience.2012.12.057

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Growing evidence has suggested that hydrogen sulfide (H2S) acts as a novel neuro-modulator and neuroprotective agent; however, it remains to be investigated whether H2S has a direct effect on neural stem cells (NSCs). We report here that NSCs expressed cystathionine p synthase (CBS) and addition of exogenous H2S donor, L-cysteine, stimulated proliferation and increased the differentiation potential of NSCs to neurons and astroglia. Moreover, pre-treatment with aminooxyacetic acid, the inhibitor of CBS or knockdown of CBS in using siRNA, significantly attenuated the effects of L-cysteine on elevated H2S levels and the cell proliferation; it also effectively suppressed L-cysteine-induced neurogenesis and astrocytogenesis. Further analysis revealed that L-cysteine-induced proliferation was associated with phosphorylation of extracellular signal-regulated kinases 1/2 and differentiation with altered expression of differentiation-related genes. Taken together, the present data suggest that L-cysteine can enhance proliferation and differentiation of NSCs via the CBS/H2S pathway, which may serve as a useful inference for elucidating its role in regulating the fate of NSCs in physiological and pathological settings. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.

引用

页码：106 / 117

页数：12

共 39 条

[1]

Abe K, 1996, J NEUROSCI, V16, P1066

[2]

Ang SF, 2012, PLOS ONE, V6

[3] Cystathionine β-synthase, a key enzyme for homocysteine metabolism, is preferentially expressed in the radial glia/astrocyte lineage of developing mouse CNS [J].