The inhibitory effect of polysaccharide from Rhizopus nigricans on colitis-associated colorectal cancer

被引：33
作者：
Song, Ge [1 ]
Lu, Yan [1 ,2 ]
Yu, Zhidan [1 ,2 ]
Xu, Lei [1 ,2 ]
Liu, Jing [1 ,2 ]
Chen, Kaoshan [1 ,2 ]
Zhang, Pengying [1 ,2 ]
机构：
[1] Shandong Univ, Natl Glycoengn Res Ctr, 72 Binhai Rd, Qingdao 266237, Peoples R China
[2] Shandong Univ, Sch Life Sci, 72 Binhai Rd, Qingdao 266237, Peoples R China
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2019年 / 112卷
关键词：
Polysaccharide; Colitis; Colitis-associated cancer; Mouse model; Antitumor effect; MOUSE COLON CARCINOGENESIS; KAPPA-B; HOMEOBOX GENE; UP-REGULATION; STEM-CELLS; APOPTOSIS; MODEL; EXOPOLYSACCHARIDE; PATHWAY; EXPRESSION;
D O I：
10.1016/j.biopha.2019.01.054
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
An extracellular polysaccharide (EPS1-1) of Rhizopus nigricans was found to enhance immunity and reduce colon cancer cell proliferation. Here, the effect of EPS1-1 on a mouse model of colitis-associated cancer (CAC) induced by azoxymethane (AOM)/dextran sodium sulfate (DSS) was investigated. Pathological symptoms, including weight loss, piloerection, hematochezia and insensitivity caused by AOM/DSS, were relieved by EPS1-1. Anatomical results showed a 100% tumor incidence, a series of neoplasms, disordered cell structure and hyperplastic glands in the model group, while the abnormal behaviors were relieved and the tumors decreased in the EPS1-1 group. Compared with the model group, the EPS1-1 group showed decreased oncogenic protein (COX-2, beta-catenin, CyclinD1 and C-Myc) expression. TUNEL staining showed that EPS1-1 increased the apoptosis of colon cancer cells in mice. Furthermore, the expression of proliferative proteins (Ki-67 and PCNA) and an antiapoptotic gene transcript (Bcl-2) were significantly down regulated by EPS1-1, while apoptotic gene transcripts (p53 and Bax) were enhanced. In addition, EPS1-1 notably decreased the number of cells positive for CD68, F4/80 and NF-kappa B and reduced the concentrations of inflammatory factors (TNF-alpha and IL-6) in serum compared with those in the model group. Taken together, these results suggest that EPS1-1 may be a therapeutic option for the prevention and treatment of CAC.
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页数：9
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