Protective effect of whey protein hydrolysates against hydrogen peroxide-induced oxidative stress on PC12 cells

被引：50
|
作者：
Zhang, Qiu-Xiang [1 ]
Ling, Yu-Fang [1 ]
Sun, Zhen [1 ]
Zhang, Li [2 ]
Yu, Hui-Xin [2 ]
Kamau, Samuel Mburu [3 ]
Lu, Rong-Rong [1 ]
机构：
[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] Minist Hlth, Jiangsu Inst Nucl Med, Key Lab Nucl Med, Jiangsu Key Lab Mol Nucl Med, Wuxi 214063, Jiangsu, Peoples R China
[3] Dairy Training Inst, Naivasha 20117, Kenya
来源：
BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 11期
关键词：
Antioxidant peptide; Hydrogen peroxide; Radical scavenging activity; PC12; cells; Reactive oxygen species; ANTIOXIDANT PROPERTIES; INDUCED APOPTOSIS; LIPID OXIDATION; SOY PROTEIN; PEPTIDES; FRACTIONS;
D O I：
10.1007/s10529-012-1017-1
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The protective effect of whey protein hydrolysates (WPHs) against H2O2-induced oxidative damage on rat pheochromocytoma line 12 (PC12) cells was studied. Whey protein was hydrolyzed by pepsin and trypsin and purified by macrospore absorption resins. PC12 cells were pretreated with WPHs (from 369 to 1,980 Da) at different concentrations for 2 h, then washed and incubated with 100 mu M H2O2 in the presence of WPHs for another 24 h. With 100-400 mu g WPH/ml the viable cells increased by 20-30 % when incubated with H2O2 suggesting that they may play a role as antioxidant in foods.
引用
收藏
页码：2001 / 2006
页数：6
相关论文
共 50 条
  • [1] Protective effect of whey protein hydrolysates against hydrogen peroxide-induced oxidative stress on PC12 cells
    Qiu-Xiang Zhang
    Yu-Fang Ling
    Zhen Sun
    Li Zhang
    Hui-Xin Yu
    Samuel Mburu Kamau
    Rong-Rong Lu
    Biotechnology Letters, 2012, 34 : 2001 - 2006
  • [2] Protective effects of wheat germ protein isolate hydrolysates (WGPIH) against hydrogen peroxide-induced oxidative stress in PC12 cells
    Zhu, Ke-Xue
    Guo, Xu
    Guo, Xiao-Na
    Peng, Wei
    Zhou, Hui-Ming
    FOOD RESEARCH INTERNATIONAL, 2013, 53 (01) : 297 - 303
  • [3] Protective effect of Homer 1a against hydrogen peroxide-induced oxidative stress in PC12 cells
    Luo, Peng
    Chen, Tao
    Zhao, Yongbo
    Xu, Haoxiang
    Huo, Kai
    Zhao, Mingming
    Yang, Yuefan
    Fei, Zhou
    FREE RADICAL RESEARCH, 2012, 46 (06) : 766 - 776
  • [4] Protective effect of trihexyphenidyl on hydrogen peroxide-induced oxidative damage in PC12 cells
    Ji, Bian-Sheng
    Gao, Yuan
    NEUROSCIENCE LETTERS, 2008, 437 (01) : 50 - 54
  • [5] Hydrogen peroxide-induced apoptosis in PC12 cells and the protective effect of puerarin
    Jiang, B
    Liu, JH
    Bao, YM
    An, LJ
    CELL BIOLOGY INTERNATIONAL, 2003, 27 (12) : 1025 - 1031
  • [6] Protective effect of Schisandrin on hydrogen peroxide-induced damage in PC12 cells
    Ren, Kuang
    Wang, Yanchun
    Fan, Hongyan
    ADVANCED ENGINEERING MATERIALS III, PTS 1-3, 2013, 750-752 : 1545 - 1548
  • [7] Protective effect of trifluoperazine on hydrogen peroxide-induced apoptosis in PC12 cells
    Liu, Shichang
    Han, Yangguang
    Zhang, Tao
    Yang, Zhuo
    BRAIN RESEARCH BULLETIN, 2011, 84 (02) : 183 - 188
  • [8] Protective Effects of Costunolide against Hydrogen Peroxide-Induced Injury in PC12 Cells
    Cheong, Chong-Un
    Yeh, Ching-Sheng
    Hsieh, Yi-Wen
    Lee, Ying-Ray
    Lin, Mei-Ying
    Chen, Chung-Yi
    Lee, Chien-Hsing
    MOLECULES, 2016, 21 (07)
  • [9] Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress and Apoptosis in PC12 Cell
    ChunKang Huang
    Yonglian Lin
    Hong Su
    Dongqing Ye
    Neurochemical Research, 2015, 40 : 27 - 35
  • [10] Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress and Apoptosis in PC12 Cell
    Huang, ChunKang
    Lin, Yonglian
    Su, Hong
    Ye, Dongqing
    NEUROCHEMICAL RESEARCH, 2015, 40 (01) : 27 - 35
12345