Quantitative analysis of hyaluronan in human synovial fluid using capillary electrophoresis

被引:36
作者
Grimshaw, J
TrochaGrimshaw, J
Fisher, W
Rice, A
Smith, S
Spedding, P
Duffy, J
Mollan, R
机构
[1] QUEENS UNIV BELFAST,DEPT CHEM ENGN,BELFAST,ANTRIM,NORTH IRELAND
[2] QUEENS UNIV BELFAST,DEPT ORTHOPAED SURG,BELFAST,ANTRIM,NORTH IRELAND
关键词
glycosaminoglycan; hyaluronan; synovial fluid; capillary electrophoresis;
D O I
10.1002/elps.1150170218
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The glycosaminoglycan, hyaluronan, can be detected in human synovial fluid by capillary electrophoresis (CE). Variations in peak shape make this technique unsuitable for quantitative analysis of hyaluronan in raw synovial fluid. Quantitative analysis was achieved by hydrolysis of the polymeric hyaluronan to the tetrasaccharide by the action of testicular hyaluronidase and separation of the product using CE. A UV detector operating at 200 nm was used. The X-ray contrast material, omnipaque, a propriety aqueous solution of iohexol was used as internal standard. A second peak in the electropherogram of synovial fluid was quantified. The variation in concentrations of these two components correlate with the arthritic disease state of a joint.
引用
收藏
页码:396 / 400
页数:5
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