Establishment of Agrobacterium-mediated genetic transformation system in Dahlia

被引：3

作者：

Otani, Yuko ^{[1
]}

Chin, Dong Poh ^{[1
]}

Mii, Masahiro ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Hort, Matsudo, Chiba 2718510, Japan

来源：

PLANT BIOTECHNOLOGY | 2013年 / 30卷 / 02期

关键词：

Agrobacterium tumefaciens; DahliaXpinnata; ornamental plant; shoot regeneration; transformation; PLANT TRANSFORMATION; TUMEFACIENS; SONICATION; TISSUE; REGENERATION; EXPRESSION; TOBACCO; SAAT; DNA; L;

D O I：

10.5511/plantbiotechnology.13.0128a

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An efficient system for Agrobacterium-mediated transformation was established in dahlia (DahliaXpinnata) 'Yamatohime'. Mass of shoot primordia (MSP) induced on MS medium supplemented with 10 mg l(-1) TDZ were inoculated with Agrobacterium tumefaciens strain EHA101 (pIG121-Hm) harboring both beta-glucuronidase (GUS) and hygromycin resistant genes. After 2 days of co-cultivation, the MSP were transferred to a selection medium containing hygromycin with meropenem for bacterial elimination. Shoots were successfully regenerated from survived MSP on hormone-free medium without hygromycin and they rooted on hormone-free medium containing hygromycin. The hygromycin-resistant plants thus obtained showed histochemical blue staining for GUS. Transformation of plants was confirmed by PCR and Southern blot analyses. Transgenic 'Kokucho' were also produced by using the same transformation procedure, suggesting wide applicability of this Agrobacterium-mediated transformation procedure for other dahlia cultivars.

引用

页码：135 / 139

页数：5

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