Attomolar Protein Detection Using in-Hole Surface Plasmon Resonance

被引：118

作者：

Ferreira, Jacqueline ^{[1
,2
]}

Santos, Marcos J. L. ^{[1
,2
]}

Rahman, Mohammad M. ^{[1
]}

Brolo, Alexandre G. ^{[1
]}

Gordon, Reuven ^{[3
]}

Sinton, David ^{[4
]}

Girotto, Emerson M. ^{[2
]}

机构：

[1] Univ Victoria, Dept Chem, Victoria, BC V8W 3V6, Canada

[2] Univ Estadual Maringa, Dept Quim, BR-87020900 Maringa, Parana, Brazil

[3] Univ Victoria, Dept Elect & Comp Engn, Victoria, BC V8W 3Z8, Canada

[4] Univ Victoria, Dept Mech Engn, Victoria, BC V8W 3P6, Canada

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

EXTRAORDINARY OPTICAL-TRANSMISSION; ENHANCED LIGHT TRANSMISSION; NANOHOLE ARRAYS; BINDING EVENTS; SENSORS; FILMS;

D O I：

10.1021/ja807704v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An in-hole nanohole surface plasmon resonance sensing scheme is demonstrated. Arrays of periodic nanoholes milled through thin layers of SiO(x) and gold were used to detect the binding of organic and biological molecules inside the nanoholes, while blocking the gold surfaces outside the holes. This new approach is more efficient than the previous nanohole array method, where the response was related to binding events taking place inside of the holes and on the top gold surface. The improved sensitivity to binding events and lower detection limit are related to resonant surface plasmon enhanced transmission through the arrays of nanoholes. The sensitivity was found to be 650nm/RIU and the detection of three attomoles of proteins was estimated from this scheme.

引用

页码：436 / +

页数：3

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