Simultaneous determination of subutinib and its active metabolite in human plasma by LC-MS/MS: Application to pharmacokinetic study

被引：1

作者：

Ding Li-kun ^{[1
]}

Yang Lin ^{[1
]}

Chen Su-ning ^{[1
]}

Li Jian-kang ^{[1
]}

Wen Ai-dong ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Pharm, Xian 710032, Shaanxi, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2015年 / 1001卷

关键词：

Subutinib; Active metabolite; LC-MS/MS; Pharmacokinetics;

D O I：

10.1016/j.jchromb.2015.07.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A selective liquid chromatographic-mass spectrometric method (LC-MS/MS) has been established and validated for simultaneous determination of subutinib and its active metabolite in human plasma. Plasma samples were extracted by liquid-liquid extraction with ethyl acetate and separated on a Wondasil C-18 (150 mm x 2.1 mm, 3.5 mu m), with methanol-0.2% formic acid solution (73:27, v/v) as mobile phase at flow rate of 0.2 ml/min. The linear range was 0.25-100 ng/mL for subutinib and 0.125-50.0 ng/mL for its active metabolite, with lower limit of quantitation of 0.25 ng/mL and 0.125 ng/mL, respectively. Intra- and inter-run precision were within 7.0 and 13.1%, and the accuracies (relative errors) were <7.0 and 8.0%, with the extraction recoveries 97.0-101.2% and 93.0-98.1% for the two analytes, respectively. The validated method was successfully applied to a pharmacokinetic study of subutinib and its active metabolite in human after oral administration of subutinib maleate capsules. (C) 2015 Published by Elsevier B.V.

引用

页码：22 / 26

页数：5