Revisiting the housekeeping genes of human placental development and insufficiency syndromes

被引：54

作者：

Drewlo, S. ^{[1
]}

Levytska, K. ^{[1
]}

Kingdom, J. ^{[1
,2
]}

机构：

[1] Univ Toronto, Ctr Res Womens & Infants Hlth, Samuel Lunenfeld Res Inst, Mt Sinai Hosp, Toronto, ON M5T 3H7, Canada

[2] Univ Toronto, Dept Obstet & Gynaecol, Mt Sinai Hosp, Toronto, ON M5T 3H7, Canada

来源：

PLACENTA | 2012年 / 33卷 / 11期

关键词：

House keeping genes; Placenta; qRTPCR; EXPRESSION; GAPDH;

D O I：

10.1016/j.placenta.2012.09.007

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gene expression analysis using semi-quantitative RT-PCR is a common tool in placental research. However the comparison of steady-state gene transcription between different clinical groups is dependent upon comparison of target mRNA data with mRNA obtained from so-called housekeeping (HK) genes whose steady-state transcription does not differ significantly between the groups. In this communication, we evaluated the performance of candidate HK genes across nine clinical groups commonly used in placental research. We used the GeNorm method to evaluate qRT-PCR data to determine the performance of candidate HKs. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：952 / 954

页数：3

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