Efficacy of a novel SARS-CoV-2 detection kit without RNA extraction and purification

被引：33

作者：

Fukumoto, Tatsuya ^{[1
]}

Iwasaki, Sumio ^{[1
]}

Fujisawa, Shinichi ^{[1
]}

Hayasaka, Kasumi ^{[1
]}

Sato, Kaori ^{[1
]}

Oguri, Satoshi ^{[1
]}

Taki, Keisuke ^{[1
]}

Nakakubo, Sho ^{[2
]}

Kamada, Keisuke ^{[2
]}

Yamashita, Yu ^{[2
]}

Konno, Satoshi ^{[2
]}

Nishida, Mutsumi ^{[1
]}

Sugita, Junichi ^{[1
]}

Teshima, Takanori ^{[1
,3
]}

机构：

[1] Hokkaido Univ Hosp, Div Lab & Transfus Med, Sapporo, Hokkaido, Japan

[2] Hokkaido Univ, Fac Med, Dept Resp Med, Sapporo, Hokkaido, Japan

[3] Hokkaido Univ, Fac Med, Dept Hematol, Sapporo, Hokkaido, Japan

来源：

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES | 2020年 / 98卷

关键词：

COVID-19; SARS-CoV-2; PCR; Saliva; SPECIMENS;

D O I：

10.1016/j.ijid.2020.06.074

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for the diagnosis of coronavirus disease 2019 (COVID-19) and preventing the spread of the virus. A novel detection kit - the 2019 Novel Coronavirus Detection Kit (nCoV-DK) - halves the detection time byeliminating the steps of RNA extraction and purification. We evaluated the concordance between the nCoV-DK and direct PCR. The virus was detected in 53/71 specimens (74.6%) by direct PCR and in 55/71 specimens (77.5%) by nCoV-DK; the overall concordance rate was 94.4%: 95.2% for nasopharyngeal swab, 95.5% for saliva, and 85.7% for sputum. The nCoV-DK test effectively detects SARS-CoV-2 in all types of sample including saliva, while reducing the time required for detection, labor, and the risk of human error. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.

引用

页码：16 / 17

页数：2

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