Autophagy proteins stabilize pathogen-containing phagosomes for prolonged MHC II antigen processing

被引：160

作者：

Romao, Susana ^{[1
]}

Gasser, Nathalie ^{[1
]}

Becker, Andrea C. ^{[3
]}

Guhl, Bruno ^{[2
]}

Bajagic, Milica ^{[1
]}

Vanoaica, Danusia ^{[1
]}

Ziegler, Urs ^{[2
]}

Roesler, Joachim ^{[5
]}

Dengjel, Joern ^{[3
,4
]}

Reichenbach, Janine ^{[6
,7
]}

Muenz, Christian ^{[1
]}

机构：

[1] Univ Zurich, Inst Expt Immunol, CH-8006 Zurich, Switzerland

[2] Univ Zurich, Ctr Microscopy & Image Anal, CH-8006 Zurich, Switzerland

[3] Univ Freiburg, Freiburg Inst Adv Studies, Sch Life Sci Life Net, D-79085 Freiburg, Germany

[4] Univ Freiburg, Ctr Biol Signalling Studies, D-79085 Freiburg, Germany

[5] Univ Clin Carl Gustav Carus Dresden, Dept Pediat, D-01307 Dresden, Germany

[6] Univ Childrens Hosp Zurich, Div Immunol Hematol Bone Marrow Transplantat, Jeffrey Modell Diagnost & Res Ctr Primary Immunod, CH-8032 Zurich, Switzerland

[7] Univ Childrens Hosp Zurich, Childrens Res Ctr, CH-8032 Zurich, Switzerland

来源：

JOURNAL OF CELL BIOLOGY | 2013年 / 203卷 / 05期

基金：

瑞士国家科学基金会;

关键词：

MATURATION; PH;

D O I：

10.1083/jcb.201308173

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Antigen preservation for presentation is a hallmark of potent antigen-presenting cells. In this paper, we report that in human macrophages and dendritic cells, a subset of phagosomes gets coated with Atg8/LC3, a component of the molecular machinery of macroautophagy, and maintains phagocytosed antigens for prolonged presentation on major histocompatibility complex class II molecules. These Atg8/LC3-positive phagosomes are formed around the antigen with TLR2 agonists and require reactive oxygen species production by NOX2 for their generation. A deficiency in the NOX2-dependent formation of these antigen storage phagosomes could contribute to compromise antifungal immune control in chronic granulomatous disease patients.

引用

页码：757 / 766

页数：10

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