Small angle neutron scattering study of the quaternary structure of haemocyanin of Rapana thomasiana

被引：2

作者：

Triolo, F

Graziano, V

Heenan, RH

机构：

[1] UNIV PALERMO,DIPARTIMENTO CHIM FIS,PALERMO,ITALY

[2] BROOKHAVEN NATL LAB,UPTON,NY 11973

[3] RUTHERFORD APPLETON LAB,CHILTON,ENGLAND

来源：

JOURNAL OF MOLECULAR STRUCTURE | 1996年 / 383卷 / 1-3期

关键词：

small angle neutron scattering; haemocyanin; quaternary structure;

D O I：

10.1016/S0022-2860(96)09294-0

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Small angle neutron scattering (SANS) experiments were performed on a series of solutions of haemocyanin (He) of Rapana thomasiana. In a first series of solutions, by changing the external contrast a model of the molecule that is apparently in disagreement with electron microscopy (EM) measurements was obtained. The apparent disagreement was resolved by performing experiments on a series of variable protein concentration solutions in D2O. The physical parameters of the model, extrapolated to zero concentration, are in excellent agreement with the EM results obtained on samples more dilute than those examined with SANS.

引用

页码：249 / 254

页数：6

共 8 条

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BEINTEMA JJ, 1990, INVERTEBRATE DIOXYGE

[2] 3-DIMENSIONAL RECONSTRUCTION OF THE ALPHA(D) AND BETA(C)-HEMOCYANINS OF HELIX-POMATIA FROM FROZEN-HYDRATED SPECIMENS [J].