PCDHB14- and GABRB1-like nervous system developmental genes are altered during early neuronal differentiation of NCCIT cells treated with ethanol

被引：14

作者：

Halder, D. ^{[1
]}

Mandal, C. ^{[1
]}

Lee, B. H. ^{[2
,3
]}

Lee, J. S. ^{[3
]}

Choi, M. R. ^{[1
]}

Chai, J. C. ^{[1
]}

Lee, Y. S. ^{[1
]}

Jung, K. H. ^{[4
]}

Chai, Y. G. ^{[1
,5
]}

机构：

[1] Hanyang Univ, Dept Mol & Life Sci, Ansan, South Korea

[2] Eulji Univ, Gangnam Eulji Hosp, Dept Psychiat, Seoul, South Korea

[3] Korean Alcohol Res Fdn, KARF Hosp, Goyang, South Korea

[4] Hanyang Univ, Inst Nat Sci & Technol, Ansan, South Korea

[5] Hanyang Univ, Dept Nanobiotechnol, Seoul 133791, South Korea

来源：

HUMAN & EXPERIMENTAL TOXICOLOGY | 2015年 / 34卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

Ethanol; folic acid; FASD; nervous system; transcriptomic; NEURAL STEM-CELLS; RETINOIC ACID; IN-VITRO; CORTICAL-NEURONS; EXPRESSION; EXPOSURE; ALCOHOL; PROTEIN; RECEPTORS; MICE;

D O I：

10.1177/0960327114566827

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Ethanol (EtOH) exposure during embryonic development causes dysfunction of the central nervous system (CNS). Here, we examined the effects of chronic EtOH on gene expression during early stages of neuronal differentiation. Human embryonic carcinoma (NCCIT) cells were differentiated into neuronal precursors/lineages in the presence or absence of EtOH and folic acid. Gene expression profiling and pathway analysis demonstrated that EtOH deregulates many genes and pathways that are involved in early brain development. EtOH exposure downregulated several important genes, such as PCDHB14, GABRB1, CTNND2, NAV3, RALDH1, and OPN5, which are involved in CNS development, synapse assembly, synaptic transmission, and neurotransmitter receptor activity. GeneGo pathway analysis revealed that the deregulated genes mapped to disease pathways that were relevant to fetal alcohol spectrum disorders (FASD, such as neurotic disorders, epilepsy, and alcohol-related disorders). In conclusion, these findings suggest that the impairment of the neurological system or suboptimal synapse formation resulting from EtOH exposure could underlie the neurodevelopmental disorders in individuals with FASD.

引用

页码：1017 / 1027

页数：11

共 42 条

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