Efficacy of clarithromycin on biofilm formation of methicillin-resistant Staphylococcus pseudintermedius

被引：27

作者：

DiCicco, Matthew ^{[1
]}

Neethirajan, Suresh ^{[1
]}

Singh, Ameet ^{[2
]}

Weese, J. Scott ^{[3
]}

机构：

[1] Univ Guelph, Sch Engn, Guelph, ON N1G 2W1, Canada

[2] Univ Guelph, Ontario Vet Coll, Dept Clin Studies, Guelph, ON N1G 2W1, Canada

[3] Univ Guelph, Ontario Vet Coll, Dept Pathobiol, Guelph, ON N1G 2W1, Canada

来源：

BMC VETERINARY RESEARCH | 2012年 / 8卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

MRSP; S; pseudintermedius; Methicillin-resistant; Therapeutic; Bacteria; Biofilm; Macrolide; Clarithromycin; COAGULASE-POSITIVE STAPHYLOCOCCI; PSEUDOMONAS-AERUGINOSA; WOUND-INFECTION; DOGS; VANCOMYCIN; AUREUS; QUANTIFICATION; COLONIZATION; AZITHROMYCIN; COMBINATION;

D O I：

10.1186/1746-6148-8-225

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Background: Surgical site infections (SSIs) caused by biofilm-forming methicillin-resistant Staphylococcus pseudintermedius (MRSP) have emerged as the most common hospital-acquired infections in companion animals. No methods currently exist for the therapeutic remediation of SSIs caused by MRSP in biofilms. Clarithromycin (CLA) has been shown to prevent biofilm formation by Staphylococcus aureus. This study aims to assess the in vitro activity of CLA in eradicating MRSP biofilm formation on various materials. Results: Quantitative assay results (P = 0.5126) suggest that CLA does not eradicate MRSP biofilm formation on polystyrene after 4 - 24 h growth periods. Scanning electron micrographs confirmed that CLA did not eradicate MRSP biofilm formed on orthopaedic implants. Conclusions: By determining the in vitro characteristics and activities of MRSP isolates alone and against antibiotics, in vitro models of biofilm related infections can be made. In vitro data suggests that CLA does not effectively eradicate S. pseudintermedius biofilms in therapeutic doses.

引用

页数：7

共 34 条

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