Eighteen novel polymorphic microsatellite loci developed from the Pere David's deer (Elaphurus davidianus)

被引：5

作者：

Wu, Hai-Long ^{[1
,2
,3
,4
]}

Ni, Xiao-Wei ^{[3
,4
]}

Zhang, Lin-Yuan ^{[5
]}

Xia, Jin-Shi ^{[5
]}

Zhong, Zhen-Yu ^{[5
]}

Zhu, Guo-Ping ^{[3
,4
]}

Wan, Qiu-Hong ^{[1
,2
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, State Conservat Ctr Gene Resources Endangered Wil, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Univ, Key Lab Conservat Genet & Reproduct Biol Endanger, Minist Educ, Hangzhou 310058, Zhejiang, Peoples R China

[3] Anhui Normal Univ, Coll Life Sci, Key Lab Biot Environm & Ecol Safety Anhui Prov, Wuhu 241000, Peoples R China

[4] Anhui Normal Univ, Key Lab Mol Evolut & Biodivers, Wuhu 241000, Peoples R China

[5] Expt Ctr Beijing Elaphurus Davidianus Zool, Beijing 100076, Peoples R China

来源：

CONSERVATION GENETICS | 2008年 / 9卷 / 06期

关键词：

Elaphurus davidianus; Genetic marker; Microsatellite; Heterozygosity; Exclusion probability;

D O I：

10.1007/s10592-008-9523-3

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Pere David's deer is a severely bottlenecked species but without showing inbreeding depression, making it essential to develop molecular markers to explore her genetic mechanism of population recovery. In this study, we isolated 18 novel polymorphic microsatellite loci from a dinucleotide-enriched library. This suit of markers presented 2-3 alleles for each locus and their observed and expected heterozygosities were 0.057-0.610 and 0.056-0.598, respectively. These new microsatellite loci had an average of 2.12 alleles and thus contributed to relatively low exclusion probabilities of parentage and paternity testing (0.768 and 0.921). However, when these loci were examined in combination with previous microsatellite markers, overall probabilities of parentage and paternity exclusion went up to 0.905 and 0.990, respectively, showing that these 26 microsatellite loci should be adopted together in future genetic analyses for this highly inbred species.

引用

页码：1679 / 1682

页数：4

共 13 条

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