The Requirement of Multiple Defense Genes in Soybean Rsv1-Mediated Extreme Resistance to Soybean mosaic virus

被引：56

作者：

Zhang, Chunquan ^{[1
]}

Grosic, Sehiza ^{[1
]}

Whitham, Steven A. ^{[1
]}

Hill, John H. ^{[1
]}

机构：

[1] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2012年 / 25卷 / 10期

基金：

美国国家科学基金会;

关键词：

TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; ELICITOR FUNCTION; EXPRESSION; HSP90; P3; SENESCENCE; MUTATIONS; SEQUENCES; VIRULENCE;

D O I：

10.1094/MPMI-02-12-0046-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Soybean mosaic virus (SMV) is a major viral pathogen of soybean. Among the three SMV resistance genes, Rsv1 mediates extreme resistance (ER) against most SMV strains, including the beta-glucuronidase-tagged G2 isolate that was previously used in studies of Rsv1. Using virus-induced gene silencing (VIGS), we screened 82 VIGS constructs to identify genes that play a role in Rsv1-mediated ER to SMV infection. The target genes included putative Rsv1 candidate genes, soybean orthologs to known defense-signaling genes, and 62 WRKY transcription factors. We identified eight VIGS constructs that compromised Rsv1-mediated resistance when the target genes were silenced, including GmEDR1, GmEDS1, GmHSP90, GmJAR1, GmPAD4, and two WRKY transcription factors. Together, our results provide new insight into the soybean signaling network required for ER against SMV.

引用

页码：1307 / 1313

页数：7

共 51 条

[11] Emergence of Rsv-resistance breaking Soybean mosaic virus isolates from Korean soybean cultivars [J].

Choi, BK ;

Koo, JM ;

Ahn, HJ ;

Yum, HJ ;

Choi, CW ;

Ryu, KH ;

Chen, P ;

Tolin, SA .

VIRUS RESEARCH, 2005, 112 (1-2) :42-51

[12] Gain of virulence on Rsv1-genotype soybean by an avirulent Soybean mosaic virus requires concurrent mutations in both P3 and HC-Pro [J].

Eggenberger, A. L. ;

Hajimorad, M. R. ;

Hill, J. H. .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2008, 21 (07) :931-936

[13] Arabidopsis SENESCENCE-ASSOCIATED GENE101 stabilizes and signals within an ENHANCED DISEASE SUSCEPTIBILITY1 complex in plant innate immunity [J].

Feys, BJ ;

Wiermer, M ;

Bhat, RA ;

Moisan, LJ ;

Medina-Escobar, N ;

Neu, C ;

Cabral, A ;

Parker, JE .

PLANT CELL, 2005, 17 (09) :2601-2613

[14] GmRAR1 and GmSGT1 Are Required for Basal, R Gene-Mediated and Systemic Acquired Resistance in Soybean [J].

Fu, Da-Qi ;

Ghabrial, Said ;

Kachroo, Aardra .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2009, 22 (01) :86-95

[15] Balanced Nuclear and Cytoplasmic Activities of EDS1 Are Required for a Complete Plant Innate Immune Response [J].

Garcia, Ana V. ;

Blanvillain-Baufume, Servane ;

Huibers, Robin P. ;

Wiermer, Marcel ;

Li, Guangyong ;

Gobbato, Enrico ;

Rietz, Steffen ;

Parker, Jane E. .

PLOS PATHOGENS, 2010, 6 (07) :1-15

[16] The Bean pod mottle virus proteinase cofactor and putative helicase are symptom severity determinants [J].

Gu, HC ;

Ghabrial, SA .

VIROLOGY, 2005, 333 (02) :271-283

[17] Adaptation of Soybean mosaic virus avirulent chimeras containing P3 sequences from virulent strains to RSV1-genotype Soybeans is mediated by mutations in HC-Pro [J].

Hajimorad, M. R. ;

Eggenberger, A. L. ;

Hill, J. H. .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2008, 21 (07) :937-946

[18] Experimental Adaptation of an RNA Virus Mimics Natural Evolution [J].

Hajimorad, M. R. ;

Wen, R. -H. ;

Eggenberger, A. L. ;

Hill, J. H. ;

Maroof, M. A. Saghai .

JOURNAL OF VIROLOGY, 2011, 85 (06) :2557-2564

[19] Strain-specific P3 of Soybean mosaic virus elicits Rsv1-mediated extreme resistance, but absence of P3 elicitor function alone is insufficient for virulence on Rsv1-genotype soybean [J].

Hajimorad, MR ;

Eggenberger, AL ;

Hill, JH .

VIROLOGY, 2006, 345 (01) :156-166

[20] Loss and gain of elicitor function of Soybean mosaic virus G7 provoking Rsv1-mediated lethal systemic hypersensitive response maps to P3 [J].

Hajimorad, MR ;

Eggenberger, AL ;

Hill, JH .

JOURNAL OF VIROLOGY, 2005, 79 (02) :1215-1222

← 1 2 3 4 5 6 →