The Arabidopsis RRM domain protein EDM3 mediates race-specific disease resistance by controlling H3K9me2-dependent alternative polyadenylation of RPP7 immune receptor transcripts

被引：20

作者：

Lai, Yan ^{[1
,2
]}

Cuzick, Alayne ^{[3
,4
]}

Lu, Xueqing M. ^{[5
]}

Wang, Jianqiang ^{[1
]}

Katiyar, Neerja ^{[6
]}

Tsuchiya, Tokuji ^{[7
]}

Le Roch, Karine ^{[5
]}

McDowell, John M. ^{[8
]}

Holub, Eric ^{[3
]}

Eulgem, Thomas ^{[1
]}

机构：

[1] Univ Calif Riverside, Inst Integrat Genome Biol, Ctr Plant Cell Biol, Dept Bot & Plant Sci, Riverside, CA 92521 USA

[2] Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Fujian, Peoples R China

[3] Univ Warwick, Sch Life Sci, Wellesbourne Campus, Warwick CV35 9EF, England

[4] Rothamsted Res, Biointeract & Crop Protect, Harpenden AL5 2JQ, Herts, England

[5] Univ Calif Riverside, Inst Integrat Genome Biol, Ctr Infect Dis & Vector Res, Dept Mol Cell & Syst Biol, Riverside, CA 92521 USA

[6] Univ Calif Riverside, Inst Integrat Genome Biol, Riverside, CA 92521 USA

[7] Nihon Univ, Coll Bioresource Sci, Fujisawa, Kanagawa 2520880, Japan

[8] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24060 USA

来源：

PLANT JOURNAL | 2019年 / 97卷 / 04期

基金：

美国国家科学基金会;

关键词：

RNA-binding protein; disease-resistance genes; histone-binding proteins; transcript processing; Hyaloperonospora arabidopsidis; DOWNY MILDEW RESISTANCE; NB-LRR GENES; PERONOSPORA-PARASITICA; SALICYLIC-ACID; DNA METHYLATION; ENCODING GENES; RECOGNITION; DEFENSE; ISOLATE; LOCUS;

D O I：

10.1111/tpj.14148

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The NLR-receptor RPP7 mediates race-specific immunity in Arabidopsis. Previous screens for enhanced downy mildew (edm) mutants identified the co-chaperone SGT1b (EDM1) and the PHD-finger protein EDM2 as critical regulators of RPP7. Here, we describe a third edm mutant compromised in RPP7 immunity, edm3. EDM3 encodes a nuclear-localized protein featuring an RNA-recognition motif. Like EDM2, EDM3 promotes histone H3 lysine 9 dimethylation (H3K9me2) at RPP7. Global profiling of H3K9me2 showed EDM3 to affect this silencing mark at a large set of loci. Importantly, both EDM3 and EDM2 co-associate in vivo with H3K9me2-marked chromatin and transcripts at a critical proximal polyadenylation site of RPP7, where they suppress proximal transcript polyadeylation/termination. Our results highlight the complexity of plant NLR gene regulation, and establish a functional and physical link between a histone mark and NLR-transcript processing.

引用

页码：646 / 660

页数：15