Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore

被引：80

作者：

Bock, Lucy J. ^{[1
]}

Pagliuca, Cinzia ^{[1
]}

Kobayashi, Norihiko ^{[2
]}

Grove, Ryan A. ^{[3
]}

Oku, Yusuke ^{[2
]}

Shrestha, Kriti ^{[3
]}

Alfieri, Claudio ^{[1
]}

Golfieri, Cristina ^{[1
]}

Oldani, Amanda ^{[4
]}

Dal Maschio, Marianna ^{[1
]}

Bermejo, Rodrigo ^{[5
]}

Hazbun, Tony R. ^{[3
]}

Tanaka, Tomoyuku U. ^{[2
]}

De Wulf, Peter

机构：

[1] European Inst Oncol, Dept Expt Oncol, I-20139 Milan, Italy

[2] Univ Dundee, Coll Life Sci, Wellcome Trust Ctr Gene Regulat & Express, Dundee DD1 5EH, Scotland

[3] Purdue Univ, Ctr Canc Res, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA

[4] FIRC Inst Mol Oncol Fdn, I-20139 Milan, Italy

[5] Univ Salamanca, CSIC, Inst Biol Func & Genom, E-37008 Salamanca, Spain

来源：

NATURE CELL BIOLOGY | 2012年 / 14卷 / 06期

基金：

英国惠康基金;

关键词：

SPINDLE CHECKPOINT; BUDDING YEAST; BI-ORIENTATION; SACCHAROMYCES-CEREVISIAE; MICROTUBULE ATTACHMENT; CELL-DIVISION; KINASE; CENTROMERE; PHOSPHATASE; MECHANICS;

D O I：

10.1038/ncb2495

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Kinetochores attach the replicated chromosomes to the mitotic spindle and orchestrate their transmission to the daughter cells. Kinetochore-spindle binding and chromosome segregation are mediated by the multi-copy KNL1(Spc105), MIS12(Mtw1) and NDC80(Ndc80) complexes that form the so-called KMN network. KMN-spindle attachment is regulated by the Aurora B-Ipl1 and MPS1(Mps1) kinases. It is unclear whether other mechanisms exist that support KMN activity during the cell cycle. Using budding yeast, we show that kinetochore protein Cnn1 localizes to the base of the Ndc80 complex and promotes a functionally competent configuration of the KMN network. Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases.

引用

页码：614 / +

页数：20

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