A proteomic approach to identify seminal plasma proteins in roosters (Gallus gallus domesticus)

被引：25

作者：

Marzoni, Margherita ^{[1
]}

Castillo, Annelisse ^{[1
]}

Sagona, Simona ^{[1
]}

Citti, Lorenzo ^{[2
]}

Rocchiccioli, Silvia ^{[2
]}

Romboli, Isabella ^{[1
]}

Felicioli, Antonio ^{[1
]}

机构：

[1] Univ Pisa, Dept Vet Sci, I-56124 Pisa, Italy

[2] CNR, Inst Clin Physiol, I-56124 Pisa, Italy

来源：

ANIMAL REPRODUCTION SCIENCE | 2013年 / 140卷 / 3-4期

关键词：

Gallus gallus; Seminal plasma; Proteomics; Gel electrophoresis; Mass spectrometry; Rooster; POLYACRYLAMIDE-GEL ELECTROPHORESIS; ODORANT-BINDING PROTEINS; CHICKEN SKELETAL-MUSCLE; FOWL SPERMATOZOA; DIFFERENTIAL EXPRESSION; SEMEN FREEZABILITY; SPERM MOBILITY; FLUID; INHIBITOR; FERTILITY;

D O I：

10.1016/j.anireprosci.2013.06.009

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Considering the interest in avian semen processing and storage, the objective of this study was to identify the domestic fowl seminal plasma proteins using two-dimensional gel electrophoresis (2-DE) and mass spectrometry MS/MS. For three times in a 4-month period, seminal plasma was obtained from semen collected from four local male chickens (Gallus gallus domesticus) and prepared for two-dimensional polyacrylamide gel electrophoresis. A total of 83 spots were detected across all gels and analyzed by MALDI-TOF/TOF. Among these spots, 17 have been successfully identified. The most intensely stained spots were recognized as serum albumin, ovotransferrin, alpha-enolase, fatty acid binding protein, thioredoxin, trypsin inhibitor CITI-1 and gallinacin-9. From these proteins, two are characteristic of avian seminal plasma, the ovotransferrin and gallinacin-9, and one is specific of the Gallus species, the chicken trypsin inhibitor CITI-1. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：216 / 223

页数：8

共 63 条

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