Magnetic separations in biotechnology

被引：179
作者：
Borlido, L. [1 ]
Azevedo, A. M. [1 ]
Roque, A. C. A. [2 ]
Aires-Barros, M. R. [1 ]
机构：
[1] Univ Tecn Lisboa, Inst Super Tecn, Ctr Biol & Chem Engn, Inst Biotechnol & Bioengn,Dept Bioengn, P-1049001 Lisbon, Portugal
[2] Univ Nova Lisboa, Fac Ciencias & Tecnol, Dept Quim, REQUIMTE, P-2829516 Caparica, Portugal
来源：
BIOTECHNOLOGY ADVANCES | 2013年 / 31卷 / 08期
关键词：
Magnetic particles; Magnetic separations; Synthesis; Purification; Automation; IRON-OXIDE NANOPARTICLES; NECROSIS-FACTOR-ALPHA; PROTEIN-A; IMMUNOMAGNETIC SEPARATION; IMMUNOGLOBULIN-G; CELLULOSE MICROSPHERES; MONOCLONAL-ANTIBODIES; AFFINITY PURIFICATION; SURFACE MODIFICATION; POLYMER NANOSPHERES;
D O I：
10.1016/j.biotechadv.2013.05.009
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Magnetic separations are probably one of the most versatile separation processes in biotechnology as they are able to purify cells, viruses, proteins and nucleic acids directly from crude samples. The fast and gentle process in combination with its easy scale-up and automation provide unique advantages over other separation techniques. In the midst of this process are the magnetic adsorbents tailored for the envisioned target and whose complex synthesis spans over multiple fields of science. In this context, this article reviews both the synthesis and tailoring of magnetic adsorbents for bioseparations as well as their ultimate application. (C) 2013 Elsevier Inc. All rights reserved.
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页码：1374 / 1385
页数：12
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