Simultaneous determination of three major lignans in rat plasma by LC-MS/MS and its application to a pharmacokinetic study after oral administration of Diphylleia sinensis extract

被引：13

作者：

Zhao, Chengliang ^{[1
]}

Zhang, Nan ^{[2
]}

He, Weiyan ^{[3
]}

Li, Rui ^{[3
]}

Shi, Dan ^{[3
]}

Pang, Li ^{[2
]}

Dong, Ning ^{[2
]}

Xu, Hong ^{[4
]}

Ji, Honglei ^{[5
]}

机构：

[1] Jilin Univ, Hosp 1, Changchun 130021, Peoples R China

[2] Jilin Univ, Dept Emergency Med, Hosp 1, Changchun 130021, Peoples R China

[3] Changchun Univ Chinese Med, Dept Emergency Med, Affiliated Hosp, Changchun 130021, Peoples R China

[4] Jilin Univ, Dept Gastroenterol, Hosp 1, Changchun 130021, Peoples R China

[5] Jilin Univ, Dept Cardiol, Hosp 1, Changchun 130021, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2014年 / 28卷 / 04期

关键词：

Diphylleia sinensis; lignan; rat plasma; LC-MS; MS; pharmacokinetics; LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; HUMAN SERUM; PODOPHYLLOTOXIN; DERIVATIVES; METABOLITE; ETOPOSIDE;

D O I：

10.1002/bmc.3066

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and selective liquid chromatography tandem mass spectrometry was developed and validated for the simultaneous determination of three major lignans (podophyllotoxin, epipodophyllotoxin, and 4-demethylpodophyllotoxin) in rat plasma using diphenhydramine as the internal standard. The analytes were detected using a triple quadrupole mass spectrometer that was equipped with an electrospray ionization source in the positive ion and selected reaction monitoring modes. The linearity of the calibration curve was good, with coefficients of determination (r(2)) >0.9914 for all of the analytes. The developed method was successfully applied for the simultaneous determination of the three lignans in rat plasma following oral administration of Diphylleia sinensis extract to rats. Copyright (c) 2013 John Wiley & Sons, Ltd.

引用

页码：463 / 467

页数：5

共 13 条

[1]

[Anonymous], 2001, Guidance for Industry: Bioanalytical Method Validation

[2] Synthesis and cytotoxicity of podophyllotoxin analogues modified in the A ring [J].

Castro, A ;

del Corral, JMM ;

Gordaliza, M ;

Grande, C ;

Gómez-Zurita, A ;

García-Grávalos, D ;

San Feliciano, A .

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2003, 38 (01) :65-74

[3]

Chattopadhyay S, 2002, J BIOSCI BIOENG, V93, P215, DOI 10.1016/S1389-1723(02)80017-2

[4] Highly sensitive liquid chromatography-electrospray mass spectrometry (LC-MS) method for the determination of etoposide levels in human serum and plasma [J].

Chen, CL ;

Uckun, FM .

JOURNAL OF CHROMATOGRAPHY B, 2000, 744 (01) :91-98

[5] Podophyllotoxin:: distribution, sources, applications and new cytotoxic derivatives [J].

Gordaliza, M ;

García, PA ;

del Corral, JMM ;

Castro, MA ;

Gómez-Zurita, MA .

TOXICON, 2004, 44 (04) :441-459

[6] In vivo immunosuppressive activity of some cyclolignans [J].

Gordaliza, M ;

Castro, MA ;

delCorral, JMM ;

LopezVazquez, ML ;

SanFeliciano, A ;

Faircloth, GT .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1997, 7 (21) :2781-2786

[7] ACTIVITY OF NK-611, A NEW EPIPODOPHYLLOTOXIN DERIVATIVE, AGAINST COLONY-FORMING-UNITS FROM FRESHLY EXPLANTED HUMAN TUMORS IN-VITRO [J].

HANAUSKE, AR ;

WUSTER, KC ;

LEHMER, A ;

ROTTER, M ;

SCHNEIDER, P ;

KAESERFROHLICH, A ;

RASTETTER, J ;

DEPENBROCK, H .

EUROPEAN JOURNAL OF CANCER, 1995, 31A (10) :1677-1681

[8] Synthesis and biological evaluation of new spin-labeled derivatives of podophyllotoxin [J].

Jin, Y ;

Chen, SW ;

Tian, X .

BIOORGANIC & MEDICINAL CHEMISTRY, 2006, 14 (09) :3062-3068

[9] Determination of a new podophyllotoxin derivative, TOP-53, and its metabolite in rat plasma and urine by high-performance liquid chromatography with electrochemical detection [J].

Kitamura, R ;

Bandoh, T ;

Tsuda, M ;

Satoh, T .

JOURNAL OF CHROMATOGRAPHY B, 1997, 690 (1-2) :283-288

[10]

Ma C., 1993, Acta Pharmaceutica Sinica, V28, P690

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