TNF-α activates human monocytes for Paracoccidioides brasiliensis killing by an H2O2-dependent mechanism

被引：35

作者：

Carmo, J. P. M. ^{[1
]}

Dias-Melicio, L. A. ^{[1
]}

Calvi, S. A. ^{[1
]}

Peracoli, M. T. S. ^{[1
]}

Soares, A. M. V. C. ^{[1
]}

机构：

[1] UNESP, Dept Microbiol & Immunol, Biosci Inst, BR-18618000 Botucatu, SP, Brazil

来源：

MEDICAL MYCOLOGY | 2006年 / 44卷 / 04期

关键词：

P; brasiliensis; monocytes; fungicidal activity; H2O2;

D O I：

10.1080/13693780500536885

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Human monocytes activated by recombinant tumor necrosis factor alpha (TNF-alpha) exhibited significant fungicidal activity on the yeast cells of a highly virulent strain of Paracoccidioides brasiliensis. This process was significantly inhibited in the presence of catalase (CAT - a scavenger of H2O2), but not in the presence of superoxide-dismutase (SOD - a scavenger of superoxide anion) or N-G-monomethyl-L- arginine (N-G-MMLA - a nitric oxide inhibitor). Furthermore, there was a direct association between the intracellular killing of the fungus and the production of H2O2 by activated cells. These results strongly suggest a role for H2O2 in the killing of highly virulent strains of P. brasiliensis by TNF-alpha-activated human monocytes.

引用

页码：363 / 368

页数：6

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