A poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336:: Gene cloning and biochemical analysis of poly-γ-glutamate produced by Escherichia coli clone cells

被引：155

作者：

Ashiuchi, M

Soda, K

Misono, H ^{[1
]}

机构：

[1] Kochi Univ, Fac Agr, Dept Bioresource Sci, Res Inst Mol Genet,Nanko Ku, Kochi 7838502, Japan

[2] Kansai Univ, Dept Biotechnol, Osaka 5648680, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 263卷 / 01期

关键词：

D O I：

10.1006/bbrc.1999.1298

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three genes encoding a poly-gamma-glutamate synthetic system of Bacillus subtilis IFO 3336 (Bacillus natto) were cloned and expressed in Escherichia coli. The E. coli clone produced poly-gamma-glutamate extracellularly. The genes, newly designated as pgsBCA, were homologous with capBCA genes of Bacillus anthracis. All of pgsB, pgsC, and pgsA genes were essential for the polymer production. Addition of Mn2+, instead of Mg2+, to the polymer-synthesis medium resulted in an increase in the polymer yield. Co-expression of glutamate racemase gene in E. coli cells harboring pgsBCA genes increased both the polymer production and D-glutamate content in the polymer. The polymer produced by the E. coli clone was higher in average molecular size than that produced by B. subtilis IFO 3336. (C) 1999 Academic Press.

引用

页码：6 / 12

页数：7

共 26 条

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