A microfluidic flow injection system for DNA assay with fluids driven by an on-chip integrated pump based on capillary and evaporation effects

被引：43

作者：

Xu, Zhang-Run ^{[1
]}

Zhong, Chong-Hui ^{[1
]}

Guan, Yan-Xia ^{[1
]}

Chen, Xu-Wei ^{[1
]}

Wang, Jian-Hua ^{[1
]}

Fang, Zhao-Lun ^{[1
]}

机构：

[1] Northeastern Univ, Res Ctr Analyt Sci, Shenyang 110004, Peoples R China

来源：

LAB ON A CHIP | 2008年 / 8卷 / 10期

关键词：

D O I：

10.1039/b805774e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A miniaturized flow injection analysis (FIA) system integrating a micropump on a microfluidic chip based on capillary and evaporation effects was developed. The pump was made by fixing a filter paper plug with a vent tube at the channel end, it requires no peripheral equipment and provides steady flow in the mu l min(-1) range for FIA operation. Valve-free sample injection was achieved at nanolitre level using an array of slotted vials. The practical applicability of the system was demonstrated by DNA assay with laser-induced fluorescence (LIF) detection. A precision of 1.6% RSD (10.0 ng mu l(-1), n = 15) was achieved with a sampling throughput of 76 h(-1) and sample consumption of 95 nl.

引用

页码：1658 / 1663

页数：6

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