Bluetongue virus serotype 27: Experimental infection of goats, sheep and cattle with three BTV-27 variants reveal atypical characteristics and likely direct contact transmission BTV-27 between goats

被引：45

作者：

Breard, E. ^{[1
]}

Schulz, C. ^{[2
,6
]}

Sailleau, C. ^{[1
]}

Bernelin-Cottet, C. ^{[3
]}

Viarouge, C. ^{[1
]}

Vitour, D. ^{[1
]}

Guillaume, B. ^{[4
]}

Caignard, G. ^{[1
]}

Gorlier, A. ^{[1
]}

Attoui, H. ^{[1
]}

Gallois, M. ^{[5
]}

Hoffmann, B. ^{[2
]}

Zientara, S. ^{[1
]}

Beer, M. ^{[2
]}

机构：

[1] Univ Paris Est, Lab Sante Anim Alfort, ANSES, ENVA,INRA,UMR VIROL 1161, Maisons Alfort, France

[2] Friedrich Loeffler Inst, Inst Diagnost Virol, Greifswald, Germany

[3] UR892 INRA, Virol & Immunol Mol, Jouy En Josas, France

[4] Univ Paris Est, Ecole Natl Vet Alfort, Unite Pathol Betail, Maisons Alfort, France

[5] Reg Federat Corsican Anim Hlth Grp, FRGDSB20, Ajaccio, France

[6] Univ Vet Med Hannover, Inst Parasitol, Hannover, Germany

来源：

TRANSBOUNDARY AND EMERGING DISEASES | 2018年 / 65卷 / 02期

关键词：

animal trial; bluetongue virus serotype; BTV-27; variants; ruminants; transmission; CLINICAL SIGNS; NETHERLANDS; VACCINE; FRANCE; OUTBREAKS; EPIDEMIC; CORSICA; EUROPE;

D O I：

10.1111/tbed.12780

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Bluetongue virus (BTV) hitherto consisted of 26 recognized serotypes, of which all except BTV-26 are primarily transmitted by certain species of Culicoides biting midges. Three variants of an additional 27th bluetongue virus serotype (BTV-27v01-v03) were recently detected in asymptomatic goats in Corsica, France, 2014-2015. Molecular characterization revealed genetic differences between the three variants. Therefore, invivo characteristics were investigated by experimental infection of a total of 15 goats, 11 sheep and 4 cattle with any one of the three variants in separated animal trials. In goat trials, BTV-naive animals of the same species were kept in a facility where direct contact was unhindered. Of the 15 inoculated goats, 13 and 14 animals were found positive for BTV-RNA and antibodies (Ab), respectively, until the end of the experiments. Surprisingly, BTV-Ab levels as measured with ELISA and neutralization test (SNT) were remarkably low in all seropositive goats. Virus isolation from whole-blood was possible at the peak of viremia until 49dpi. Moreover, detection of BTV-27v02-RNA and Ab in one contact goat indicated thatsimilar to BTV-26at least one of three BTV-27 variants may be transmitted by contact between goats. In the field, BTV-27 RNA can be detected up to 6months in the whole-blood of BTV-27-infected Corsican goats. In contrast, BTV RNA was not detected in the blood of cattle or sheep. In addition, BTV-27 Abs were not detected in cattle and only a transient increase in Ab levels was observed in some sheep. None of the 30 animals showed obvious BT-like clinical signs. In summary, the phenotypes observed for BTV-27v01-v03 phenotypes correspond to a mixture of characteristics known for BTV-25 and 26.

引用

页码：e251 / e263

页数：13

共 43 条

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