An improved fluorimetric HPLC method for quantifying tocopherols in Brassica rapa L. subsp sylvestris after harvest

被引：25

作者：

Annunziata, Maria Grazia ^{[1
]}

Attico, Assunta ^{[1
]}

Woodrow, Pasqualina ^{[1
]}

Oliva, Maria Alessandra ^{[1
]}

Fuggi, Amodio ^{[1
]}

Carillo, Petronia ^{[1
]}

机构：

[1] Univ Naples 2, Dipartimento Sci Vita, I-81100 Caserta, Italy

来源：

JOURNAL OF FOOD COMPOSITION AND ANALYSIS | 2012年 / 27卷 / 02期

关键词：

Brassica rapa; alpha-Tocopherol; gamma-Tocopherol; Post-harvest; Ascorbate; Dehydroascorbate; Fluorimetric HPLC method; Tocopherol determination; Food analysis; Food composition; PERFORMANCE LIQUID-CHROMATOGRAPHY; ALPHA-TOCOPHEROL; VITAMIN-E; BROCCOLI; PLANTS; CAROTENOIDS; ASCORBATE;

D O I：

10.1016/j.jfca.2012.05.006

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study describes a reliable method suitable for the quantitative determination of tocopherols with a fast, easy extraction procedure and a rapid, sensitive HPLC fluorescence detection, that results in a better signal-to-noise ratio and lowers the detection limit. The limit of detection of this assay is about 0.06 mg L-1 and the linearity is in a range of 0.06-2 mg L-1. The method has been tested on samples of Brassica rapa L. subsp. sylvestris after harvest as affected by various storage temperatures (4 degrees C, 9 degrees C and 20 degrees C). The fluorescent detection makes it possible to measure tocopherols in these plants even when their concentration is extremely low, as at harvest, without the interference of other metabolites. The increase of tocopherols was followed for 14 days after harvest, and in particular the metabolic profile of a-tocopherol was compared with that of ascorbate, dehydroascorbate and chlorophyll. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：145 / 150

页数：6

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