Chloroplast Biogenesis Is Regulated by Direct Action of the Ubiquitin-Proteasome System

被引：208

作者：

Ling, Qihua ^{[1
]}

Huang, Weihua ^{[1
]}

Baldwin, Amy ^{[1
]}

Jarvis, Paul ^{[1
]}

机构：

[1] Univ Leicester, Dept Biol, Leicester LE1 7RH, Leics, England

来源：

SCIENCE | 2012年 / 338卷 / 6107期

基金：

英国生物技术与生命科学研究理事会;

关键词：

MITOCHONDRIAL E3; IMPORT RECEPTOR; ARABIDOPSIS; LIGASE; PROTEINS; PLASTIDS; DEGRADATION; PATHWAYS; DYNAMICS; NUCLEUS;

D O I：

10.1126/science.1225053

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Development of chloroplasts and other plastids depends on the import of thousands of nucleus-encoded proteins from the cytosol. Import is initiated by TOC (translocon at the outer envelope of chloroplasts) complexes in the plastid outer membrane that incorporate multiple, client-specific receptors. Modulation of import is thought to control the plastid's proteome, developmental fate, and functions. Using forward genetics, we identified Arabidopsis SP1, which encodes a RING-type ubiquitin E3 ligase of the chloroplast outer membrane. The SP1 protein associated with TOC complexes and mediated ubiquitination of TOC components, promoting their degradation. Mutant sp1 plants performed developmental transitions that involve plastid proteome changes inefficiently, indicating a requirement for reorganization of the TOC machinery. Thus, the ubiquitin-proteasome system acts on plastids to control their development.

引用

页码：655 / 659

页数：5

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