Cadmium-induced DNA damage and mutations in Arabidopsis plantlet shoots identified by DNA fingerprinting

被引：38
作者：
Liu, Wan [1 ]
Sun, Lizong [1 ]
Zhong, Ming [2 ]
Zhou, Qixing [3 ]
Gong, Zongqiang [1 ]
Li, Peijun [1 ]
Tai, Peidong [1 ]
Li, Xiaojun [1 ]
机构：
[1] Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China
[2] Shenyang Agr Univ, Coll Biol Sci & Technol, Shenyang 110866, Peoples R China
[3] Nankai Univ, Coll Environm Sci & Engn, Key Lab Pollut Proc & Environm Criteria, Minist Educ, Tianjin 300071, Peoples R China
来源：
CHEMOSPHERE | 2012年 / 89卷 / 09期
基金：
中国国家自然科学基金;
关键词：
RAPD; DNA damage and mutations; Cd; Biomarker; Arabidopsis thaliana; IN-VITRO; RAPD; PHOSPHOGLUCOMUTASE; SLEEPY1; GENE; CARCINOGENESIS; CONTAMINATION; BIOSYNTHESIS; GENOTOXICITY; POPULATION;
D O I：
10.1016/j.chemosphere.2012.05.068
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Random amplified polymorphic DNA (RAPD) test is a feasible method to evaluate the toxicity of environmental pollutants on vegetal organisms. Herein, Arabidopsis thaliana (Arabidopsis) plantlets following Cadmium (Cd) treatment for 26 d were screened for DNA genetic alterations by DNA fingerprinting. Four primers amplified 20-23 mutated RAPD fragments in 0.125-3.0 mg L-1 Cd-treated Arabidopsis plantlets, respectively. Cloning and sequencing analysis of eight randomly selected mutated fragments revealed 99-100% homology with the genes of VARICOSE-Related, SLEEPY1 F-box, 40S ribosomal protein S3, phosphoglucomutase, and noncoding regions in Arabidopsis genome correspondingly. The results show the ability of RAPD analysis to detect significant genetic alterations in Cd-exposed seedlings. Although the exact functional importance of the other mutated bands is unknown, the presence of mutated loci in Cd-treated seedlings, prior to the onset of significant physiological effects, suggests that these altered loci are the early events in Cd-treated Arabidopsis seedlings and would greatly improve environmental risk assessment. (c) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1048 / 1055
页数：8
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