A Novel Chemiluminescent Probe Based on 1,2-Dioxetane Scaffold for Imaging Cysteine in Living Mice

被引：42
|
作者：
Sun, Jinyu [1 ]
Hu, Zhian [1 ]
Zhang, Sichun [1 ]
Zhang, Xinrong [1 ]
机构：
[1] Tsinghua Univ, Beijing Key Lab Microanalyt Methods & Instrumenta, Dept Chem, Beijing 100084, Peoples R China
来源：
ACS SENSORS | 2019年 / 4卷 / 01期
关键词：
chemiluminescent probe; cysteine; 1,2-dioxetane; living animal; chemiluminescent imaging; in vivo imaging; REAL-TIME ANALYSIS; FLUORESCENT-PROBE; MURINE MODEL; BIOLUMINESCENCE; DESIGN;
D O I：
10.1021/acssensors.8b00936
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel chemiluminescent probe for detection of cysteine (Cys) from other biothiols has been reported by utilizing the excellent chemiluminescent Schaap's adamantylidene-dioxetane scaffold. After careful assessment, the probe CL-Cys could detect Cys with high sensitivity and total light photons increased by 28-fold after the probe was treated with Cys, with the detection limit of 7.5 X 10(-8) M. Finally, CL-Cys was further utilized for the chemiluminescent imaging of endogenous Cys in a living mouse. In general, this probe has a remarkable ability to detect Cys, which provides a valuable method for interrogation of the Cys roles in more biological and pathological processes.
引用
收藏
页码：87 / 92
页数：11
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