Disposition of firocoxib in equine plasma after an oral loading dose and a multiple dose regimen

The objective of this study was to determine if a single loading dose (LD), 3x the label dose of firocoxib oral paste, followed by nine maintenance doses at the current label dose achieves and maintains near steady state concentrations. Six healthy, adult mares were administered 0.3 mg/kg of firocoxib on Day 0, and 0.1 mg/kg 24 h later on Day 1, and at 24 h intervals from Day 2 to Day 9, for a total of 10 doses. Blood samples were collected throughout the study. The mean firocoxib maximum plasma concentration and standard deviation was 199 +/- 97 ng/mL, 175 +/- 44 ng/mL and 183 +/- 50 ng/mL after the LD, and first and last maintenance doses, respectively. The minimum mean concentration (C-min) increased from 100 +/- 23 ng/mL after the LD to 132 +/- 38 ng/mL at Day 7. Then, the C-min remained constant until Day 9. The average concentration at steady state (Cavg) was 150 +/- 45 ng/mL, which compares well to the C-avg (130 +/- 36 ng/mL) reported after multiple daily doses at 0.1 mg/kg. The administration of the single LD allowed achievement of the average steady state drug concentrations faster than a multi-dose regimen without a loading dose. After the LD, firocoxib at 0.1 mg/kg every 24 h was able to maintain a relatively constant average drug concentration which should produce less variability in onset of action and efficacy. (C) 2013 Elsevier Ltd. All rights reserved.