Analyzing pH-induced changes in a myofibril model system with vibrational and fluorescence spectroscopy

被引：22

作者：

Andersen, Petter Vejle ^{[1
]}

Veiseth-Kent, Eva ^{[1
]}

Wold, Jens Petter ^{[1
]}

机构：

[1] Nofima AS, Osloveien 1, N-1430 As, Norway

来源：

MEAT SCIENCE | 2017年 / 125卷

关键词：

Water-holding capacity; pH; Proteins; Vibrational spectroscopy; Fluorescence; WATER-HOLDING CAPACITY; MEAT QUALITY TRAITS; RAMAN-SPECTROSCOPY; EARLY POSTMORTEM; DRIP LOSS; PORCINE MEAT; SIDE-CHAINS; PORK; PROTEINS; PREDICTION;

D O I：

10.1016/j.meatsci.2016.11.005

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The decline of pH and ultimate pH in meat postmortem greatly influences meat quality (e.g. water holding capacity). Four spectroscopic techniques, Raman, Fourier transform infrared (FT-IR), near infrared (NIR) and fluorescence spectroscopy, were used to study protein and amino acid modifications to determine pH-related changes in pork myofibril extracts at three different pH-levels, 53, 5.8 and 63. Protonation of side-chain carboxylic acids of aspartic and glutamic acid and changes in secondary structure, mainly the amide I-Ill peaks, were the most important features identified by Raman and FT-IR spectroscopy linked to changes in pH. Fluorescence spectroscopy identified tryptophan interaction with the molecular environment as the most important contributor to changes in the spectra. NIR spectroscopy gave no significant contributions to interpreting protein structure related to pH. Results from our study are useful for interpreting spectroscopic data from meat where pH is an important variable. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：1 / 9

页数：9

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[11] Prediction of water-holding capacity and composition of porcine meat by comparative spectroscopy [J].