Antioxidant Rich Morus alba leaf Extract Induces Apoptosis in Human Colon and Breast Cancer Cells by the Downregulation of Nitric Oxide Produced by Inducible Nitric Oxide Synthase

被引:72
作者
Deepa, Mundekkad [1 ]
Sureshkumar, Thavamani [1 ]
Satheeshkumar, Padikara Kutty [1 ]
Priya, Sulochana [1 ]
机构
[1] VIT Univ, Ctr Bioseparat Technol, Vellore 632014, Tamil Nadu, India
来源
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL | 2013年 / 65卷 / 02期
关键词
L; EXPRESSION; LEAVES;
D O I
10.1080/01635581.2013.748924
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Morus species had been used widely in the traditional medicines for various diseases. In this study we report the in vitro antiproliferative activity of the methanol extract of Morus alba. The extract is capable of inducing cytotoxicity in human colon cancer (HCT-15) cells (IC50 = 13.8g/ml) and breast cancer (MCF-7) cells (IC50= 9.2g/ml), resulted in significant morphological changes of the cells, fragmentation of DNA, and caspase-3 activation- characteristics of apoptosis. It downregulated the amount of nitric oxide (NO) produced as a result of inducible nitric oxide (iNOS) activation. The HPLC analysis of the extract showed epicatechin (20%), myricetin (10%), quercetin hydrate (12%), luteolin (12%), and kaempferol (6%) as the major active components and ascorbic acid, gallic acid, pelargonidine, and p-coumaric acid as the minor components. To the best of our knowledge, this is the first report showing the downregulation of iNOS and induction of apoptosis by M. alba extract.
引用
收藏
页码:305 / 310
页数:6
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