Role of Alternative Polyadenylation during Adipogenic Differentiation: An In Silico Approach

被引：11
作者：
Spangenberg, Lucia [1 ]
Correa, Alejandro [2 ]
Dallagiovanna, Bruno [2 ]
Naya, Hugo [1 ,3 ]
机构：
[1] Inst Pasteur Montevideo, Bioinformat Unit, Montevideo, Uruguay
[2] Fiocruz Parana, Inst Carlos Chagas, Curitiba, Parana, Brazil
[3] Univ Republ, Dept Prod Anim & Pasturas, Fac Agron, Curitiba, Parana, Brazil
来源：
PLOS ONE | 2013年 / 8卷 / 10期
关键词：
MESENCHYMAL STEM-CELLS; 3' UNTRANSLATED REGIONS; MESSENGER-RNAS; EXPRESSION; MICRORNAS; MOUSE; TRANSCRIPTOME; PLURIPOTENCY; DETERMINANTS; NETWORKS;
D O I：
10.1371/journal.pone.0075578
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Post-transcriptional regulation of stem cell differentiation is far from being completely understood. Changes in protein levels are not fully correlated with corresponding changes in mRNAs; the observed differences might be partially explained by post-transcriptional regulation mechanisms, such as alternative polyadenylation. This would involve changes in protein binding, transcript usage, miRNAs and other non-coding RNAs. In the present work we analyzed the distribution of alternative transcripts during adipogenic differentiation and the potential role of miRNAs in post-transcriptional regulation. Our in silico analysis suggests a modest, consistent, bias in 3'UTR lengths during differentiation enabling a fine-tuned transcript regulation via small non-coding RNAs. Including these effects in the analyses partially accounts for the observed discrepancies in relative abundance of protein and mRNA.
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页数：13
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