Bombarding Cancer: Biolistic Delivery of therapeutics using Porous Si Carriers

被引：30
作者：
Zilony, Neta [1 ,2 ]
Tzur-Balter, Adi [3 ,4 ]
Segal, Ester [4 ,5 ]
Shefi, Orit [1 ,2 ]
机构：
[1] Bar Ilan Univ, Fac Engn, IL-5290002 Ramat Gan, Israel
[2] Bar Ilan Univ, Bar Ilan Inst Nanotechnol & Adv Mat, IL-52900 Ramat Gan, Israel
[3] Technion Israel Inst Technol, Interdept Program Biotechnol, IL-32000 Haifa, Israel
[4] Technion Israel Inst Technol, Dept Biotechnol & Food Engn, IL-32000 Haifa, Israel
[5] Technion Israel Inst Technol, Russell Berrie Nanotechnol Inst, IL-32000 Haifa, Israel
来源：
SCIENTIFIC REPORTS | 2013年 / 3卷
基金：
欧盟第七框架计划;
关键词：
MESOPOROUS SILICA NANOPARTICLES; DRUG-DELIVERY; RELEASE; MICROPARTICLES; TRANSFECTION; TISSUES; SYSTEM; CELLS; MODEL; GUN;
D O I：
10.1038/srep02499
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A new paradigm for an effective delivery of therapeutics into cancer cells is presented. Degradable porous silicon carriers, which are tailored to carry and release a model anti-cancer drug, are biolistically bombarded into in-vitro cancerous targets. We demonstrate the ability to launch these highly porous microparticles by a pneumatic capillary gene gun, which is conventionally used to deliver cargos by heavy metal carriers. By optimizing the gun parameters e.g., the accelerating gas pressure, we have successfully delivered the porous carriers, to reach deep targets and to cross a skin barrier in a highly spatial resolution. Our study reveals significant cytotoxicity towards the target human breast carcinoma cells following the delivery of drug-loaded carriers, while administrating empty particles results in no effect on cell viability. The unique combination of biolistics with the temporal control of payload release from porous carriers presents a powerful and non-conventional platform for designing new therapeutic strategies.
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页数：6
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