Clonal propagation of Rosa clinophylla Thory. through axillary bud culture

A protocol for clonal propagation of Rosa clinophylla, a rare and endangered species but very important for breeding purposes had been standardized through in vitro axillary bud culture. Although cytokinins alone were able to induce shoot buds, but their proper growth and number could be increased only when they were used in combination with GA(3). However, there was shoot tip necrosis and leaf fall in the proliferated shoots. AgNO3 at 58.85 mu M proved effective to avoid shoot necrosis and yellowing of leaves. Activated charcoal (AC) at 250 mg l(-1) was found necessary at all the stages of shoot multiplication as well as rooting. Ninety percent rooting could be achieved in 1/2 MS medium supplemented with 4.92 mu M IBA and 250 mg l(-1) AC. Rooted plantlets were hardened and transferred to the field successfully with 80% survival rate. (C) 2008 Elsevier B.V. All rights reserved.