Angiogenic effects of dual gene transfer of bFGF and PDGF-BB after myocardial infarction

被引：49
作者：
Hao, X
Månsson-Broberg, A
Gustafsson, T
Grinnemo, KH
Blomberg, P
Siddiqui, AJ
Wärdell, E
Sylvén, C
机构：
[1] Huddinge Univ Hosp, Dept Cardiol, Karolinska Inst, SE-14186 Stockholm, Sweden
[2] Novum, Gene Therapy Ctr, Stockholm, Sweden
[3] Karolinska Univ Hosp Huddinge, Dept Thorax Surg, Stockholm, Sweden
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 315卷 / 04期
关键词：
angiogenesis; arteriogenesis; bFGF; PDGF-BB; plasmid; myocardial infarction;
D O I：
10.1016/j.bbrc.2004.01.165
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Therapeutic effects of combination of angiogenic growth factors for the treatment of ischemia after myocardial infarction are largely unknown. Plasmids expressing basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF-BB) or their combination with a 1:1 mass ratio were injected into hearts with 7-day-old myocardial infarction. Hearts were harvested after 1 and 4 weeks after gene transfer. The major findings in this chronic myocardial infarction model were that bFGF, PDGF-BB and their combination all had a more pronounced angiogenic effect on the arteriolar than the capillary level. bFGF stimulated both capillary and arteriolar growth while PDGF-BB preferentially stimulated arterioles. The combination increased the amount of both capillaries and arterioles and in addition gave rise to stable capillaries compared to single factor transfer but did not further enhance angiogenesis. No cardiovascular side effects were observed after gene transfer. (C) 2004 Elsevier Inc. All rights reserved.
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收藏
页码：1058 / 1063
页数：6
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