Characterization of Denitrification Gene Clusters of Soil Bacteria via a Metagenomic Approach

被引：51

作者：

Demaneche, Sandrine ^{[1
]}

Philippot, Laurent ^{[2
,3
]}

David, Maude M. ^{[1
]}

Navarro, Elisabeth ^{[1
,4
]}

Vogel, Timothy M. ^{[1
]}

Simonet, Pascal ^{[1
]}

机构：

[1] Univ Lyon, Ecole Cent Lyon, Environm Microbial Genom Grp, CNRS,Lab AMPERE,UMR 5005, F-69134 Ecully, France

[2] INRA, UMR 1229, F-21000 Dijon, France

[3] Univ Burgundy, UMR 1229, F-21000 Dijon, France

[4] Univ Montpellier 2, INRA, AGRO Montpellier, CIRAD,IRD,UMR 113,Lab Symbioses Tropicales & Medi, F-34398 Montpellier 5, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 02期

关键词：

DENITRIFYING PSEUDOMONAS-STUTZERI; NITROUS-OXIDE REDUCTASE; PHYLOGENETIC ANALYSIS; NITRIC-OXIDE; NOSZ GENES; FAMILY; NIRK; COMMUNITY; DOMAINS; PCR;

D O I：

10.1128/AEM.01706-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters.

引用

收藏

页码：534 / 537

页数：4

相关论文

共 20 条

[1] A new nos gene downstream from nosDFY is essential for dissimilatory reduction of nitrous oxide by Rhizobium (Sinorhizobium) meliloti [J].

Chan, YK ;

McCormick, WA ;

Watson, RJ .

MICROBIOLOGY-SGM, 1997, 143 :2817-2824

[2] Evaluation of functional gene enrichment in a soil metagenomic clone library [J].

Demaneche, Sandrine ;

David, Maude M. ;

Navarro, Elisabeth ;

Simonet, Pascal ;

Vogel, Timothy M. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2009, 76 (01) :105-107

[3] Presence of two different active nirS nitrite reductase genes in a denitrifying Thauera sp from a high-nitrate-removal-rate reactor [J].

Etchebehere, C ;

Tiedje, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (09) :5642-5645

[4] Phylogenetic analysis of polyketide synthase I domains from soil metagenomic libraries allows selection of promising clones [J].

Ginolhac, A ;

Jarrin, C ;

Gillet, B ;

Robe, P ;

Pujic, P ;

Tuphile, K ;

Bertrand, H ;

Vogel, TM ;

Perrière, G ;

Simonet, P ;

Nalin, R .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (09) :5522-5527

[5] Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR [J].

Henry, S ;

Baudoin, E ;

López-Gutiérrez, JC ;

Martin-Laurent, F ;

Brauman, A ;

Philippot, L .

JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 59 (03) :327-335

[6] Quantitative detection of the nosZ gene, encoding nitrous oxide reductase, and comparison of the abundances of 16S rRNA, narG, nirK, and nosZ genes in soils [J].

Henry, S. ;

Bru, D. ;

Stres, B. ;

Hallet, S. ;

Philippot, L. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (08) :5181-5189

[7] Characterization of nirV and a gene encoding a novel pseudoazurin in Rhodobacter sphaeroides 2.4.3 [J].

Jain, R ;

Shapleigh, JP .

MICROBIOLOGY-SGM, 2001, 147 :2505-2515

[8] Phylogenetic analysis of nitrite, nitric oxide, and nitrous oxide respiratory enzymes reveal a complex evolutionary history for denitrification [J].

Jones, Christopher M. ;

Stres, Blaz ;

Rosenquist, Magnus ;

Hallin, Sara .

MOLECULAR BIOLOGY AND EVOLUTION, 2008, 25 (09) :1955-1966

[9] The magic and menace of metagenomics: prospects for the study of plant growth-promoting rhizobacteria [J].

Leveau, Johan H. J. .

EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2007, 119 (03) :279-300

[10] Accurate phylogenetic classification of variable-length DNA fragments [J].

McHardy, Alice Carolyn ;

Garcia Martin, Hector ;

Tsirigos, Aristotelis ;

Hugenholtz, Philip ;

Rigoutsos, Isidore .

NATURE METHODS, 2007, 4 (01) :63-72