Proteome of the bacterium Mycoplasma penetrans

被引：21
作者：
Ferrer-Navarro, M
Gómez, A
Yanes, O
Planell, R
Avilés, FX
Piñol, J
Pons, JAP
Querol, E [1 ]
机构：
[1] Univ Autonoma Barcelona, Inst Biotecnol & Biomed, E-08193 Barcelona, Spain
[2] Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, E-08193 Barcelona, Spain
来源：
JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 03期
关键词：
proteomics; mass spectrometry; two-dimensional gel electrophoresis; phosphoproteome; genome annotation;
D O I：
10.1021/pr050340p
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A proteome map of Mycoplasma penetrans has been constructed using two-dimensional gel electrophoresis in combination with mass spectrometry (MS). Mycoplasma penetrans infects the urogenital and respiratory tracts of humans. A total of 207 spots were characterized with MS and, in comparing the experimental data with the DNA sequence-derived predictions, it was possible to assign these 207 spots to 153 genes. The Pro-Q Diamond phosphoprotein dye technology was used for the fluorescent detection of 26 phosphoproteins in the 4-7 pH range.
引用
收藏
页码：688 / 694
页数：7
相关论文
共 35 条
[1]   PHOSPHORYLATION OF ELONGATION-FACTOR TU PREVENTS TERNARY COMPLEX-FORMATION [J].
ALEXANDER, C ;
BILGIN, N ;
LINDSCHAU, C ;
MESTERS, JR ;
KRAAL, B ;
HILGENFELD, R ;
ERDMANN, VA ;
LIPPMANN, C .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (24) :14541-14547
[2]  
Aloy P, 1997, COMPUT APPL BIOSCI, V13, P231
[3]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[4]   Relation between amino acid composition and cellular location of proteins [J].
Cedano, J ;
Aloy, P ;
PerezPons, JA ;
Querol, E .
JOURNAL OF MOLECULAR BIOLOGY, 1997, 266 (03) :594-600
[5]   Evidence for specific and non-covalent binding of lipids to natural and recombinant Mycobacterium bovis BCG Hsp60 proteins, and to the Escherichia coli homologue GroEL [J].
De Bruyn, J ;
Soetaert, K ;
Buyssens, P ;
Calonne, I ;
De Coene, JL ;
Gallet, X ;
Brasseur, R ;
Wattiez, R ;
Falmagne, P ;
Montrozier, H ;
Lanéelle, MA ;
Daffé, M .
MICROBIOLOGY-SGM, 2000, 146 :1513-1524
[6]   Stability of cytadherence-related proteins P140/P110 in Mycoplasma genitalium requires MG218 and unidentified factor [J].
Dhandayuthapani, S ;
Rasmussen, WG ;
Baseman, JB .
ARCHIVES OF MEDICAL RESEARCH, 2002, 33 (01) :1-5
[7]   Detecting remotely related proteins by their interactions and sequence similarity [J].
Espadaler, J ;
Aragüés, R ;
Eswar, N ;
Marti-Renom, MA ;
Querol, E ;
Avilés, FX ;
Sali, A ;
Oliva, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (20) :7151-7156
[8]   Current two-dimensional electrophoresis technology for proteomics [J].
Görg, A ;
Weiss, W ;
Dunn, MJ .
PROTEOMICS, 2004, 4 (12) :3665-3685
[9]   In vivo activity of enzymatic and regulatory components of the phosphoenolpyruvate: sugar phosphotransferase system in Mycoplasma pneumoniae [J].
Halbedel, S ;
Hames, C ;
Stülke, J .
JOURNAL OF BACTERIOLOGY, 2004, 186 (23) :7936-7943
[10]   Multiple promoter inversions generate surface antigenic variation in Mycoplasma penetrans [J].
Horino, A ;
Sasaki, Y ;
Sasaki, T ;
Kenri, T .
JOURNAL OF BACTERIOLOGY, 2003, 185 (01) :231-242
1234