Preparation and Bioactivities of Electrospun PBS Nanofiber Membrane and Hybrid PBS Nanofiber Membrane of Sustained Release Growth Factors in Platelet

Poly(butylene succinate) (PBS) nanofiber membrane and hybrid nanofiber membrane of PBS and platelet-rich plasma(PRP) were prepared by electrospinning. The properties and bioactivities of scaffold materials were evaluated by means of scanning electron microscope (SEM), methyl thiazolyl tetrazolium (MTT), fluorescent staining and laser scanning co-focal microscope, hemolytic properties of materials and enzyme-linked immunosorbent assay(ELISA). The experiment results show that nanofiber membranes can accelerate the proliferation of human osteosarcoma MG63 cells line. Also, in vitro the growth factors in the scaffold material display a sustained release with the scaffold degrading after the initial burst release. In addition, hemolytic rate of scaffold material is 0.1475% (<5%), which meets the demand of hemolytic rate of medial materials.