Trypanosome mitochondrial 3′ terminal uridylyl transferase (TUTase):: The key enzyme in U-insertion/deletion RNA editing

被引：110
作者：
Aphasizhev, R
Sbicego, S
Peris, M
Jang, SH
Aphasizheva, I
Simpson, AM
Rivlin, A
Simpson, L [1 ]
机构：
[1] Univ Calif Los Angeles, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90095 USA
[3] Taegu Univ, Dept Mol Biol, Kyung San 712714, Kyung Pook, South Korea
[4] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[5] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA
来源：
CELL | 2002年 / 108卷 / 05期
关键词：
D O I：
10.1016/S0092-8674(02)00647-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A 3' terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3' U-addition in the presence of Mg2+. TUTase is present in vivo in at least two stable configurations: one contains a similar to500 kDa TUTase oligomer and the other a similar to700 kDa TUTase complex. Anti-TUTase antiserum specifically coprecipitates a small portion of the p45 and p50 RNA ligases and approximately 40% of the guide RNAs. Inhibition of TUTase expression in procyclic T. brucei by RNAi downregulates RNA editing and appears to affect parasite viability.
引用
收藏
页码：637 / 648
页数：12
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