Cytotoxic and apoptotic effects of constituents from Millettia pachycarpa Benth

被引：59

作者：

Ye, Haoyu ^{[1
]}

Fu, Afu ^{[2
]}

Wu, Wenshuang ^{[1
]}

Li, Yanfang ^{[3
]}

Wang, Guangcheng ^{[1
]}

Tang, Minghai ^{[1
]}

Li, Shucai ^{[1
]}

He, Shichao ^{[1
]}

Zhong, Shijie ^{[1
]}

Lai, Huijun ^{[1
]}

Yang, Jianhong ^{[1
]}

Xiang, Minli ^{[1
]}

Peng, Aihua ^{[1
]}

Chen, Lijuan ^{[1
]}

机构：

[1] Sichuan Univ, W China Med Sch, W China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China

[2] Nanyang Technol Univ, Sch Chem & Biomol Engn, Singapore 639798, Singapore

[3] Sichuan Univ, Sch Chem Engn, Chengdu 610041, Peoples R China

来源：

FITOTERAPIA | 2012年 / 83卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Millettia pachycarpa Benth; Separation; Millepachine; Cytotoxicity; Apoptosis; COUNTER-CURRENT CHROMATOGRAPHY; COLON-CANCER CELLS; FLAVONOIDS; ISOFLAVONES; DERIVATIVES; BARBIGERONE; INSECTICIDE; FERRUGINEA; ROTENOIDS; TEPHROSIN;

D O I：

10.1016/j.fitote.2012.08.001

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The aim of this study is to investigate the cytotoxic and apoptotic effects of constituents from the seeds of Millettia pachycarpa Benth. Fourteen compounds (1-14) including one novel chalcone (10) were isolated as active principles from Chinese herbal medicine M. pachycarpa Benth. Their structures were identified by using spectroscopic methods. All isolates were then evaluated for their cytotoxic effects against several cancer cell lines (HepG2, (26, LL2 and B16) with cisplatin as a positive control. And their apoptosis-inducing effects were tested against HeLa-C3 cells with taxol as a positive control. Both studies showed that compounds 1, 2, 7 and 10 demonstrated significant cytotoxic and apoptotic effects against cancer cells. Moreover, in the apoptosis assay the novel chalcone (10) showed strong apoptosis inducing effects at a concentration of 2 mu M within 36 h. It was found to be the most potent a poptotic inducer of the compounds isolated from M. pachycarpa Benth. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：1402 / 1408

页数：7

共 38 条

[31] Cytotoxic activity of a flavanone from the stem of Bauhinia variegata Linn [J].

Rajkapoor, Balasubramanian ;

Murugesh, Narayanan ;

Krishna, Devarakonda Rama .

NATURAL PRODUCT RESEARCH, 2009, 23 (15) :1384-1389

[32] Session 1: Antioxidants and the immune system Flavonoids as anti-inflammatory agents [J].

Serafini, Mauro ;

Peluso, Ilaria ;

Raguzzini, Anna .

PROCEEDINGS OF THE NUTRITION SOCIETY, 2010, 69 (03) :273-278

[33] A CHALCONE AND AN ISOFLAVONE FROM MILLETTIA-PACHYCARPA SEEDS [J].

SINGHAL, AK ;

BARUA, NC ;

SHARMA, RP ;

BARUAH, JN .

PHYTOCHEMISTRY, 1983, 22 (04) :1005-1006

[34] NEW ROTENOIDS IN AMORPHA-FRUTICOSA FRUITS [J].

SOMLEVA, T ;

OGNYANOV, I .

PLANTA MEDICA, 1985, 51 (03) :219-221

[35] Aminoflavone, a Ligand of the Aryl Hydrocarbon Receptor, Inhibits HIF-1α Expression in an AhR-Independent Fashion [J].

Terzuoli, Erika ;

Puppo, Maura ;

Rapisarda, Annamaria ;

Uranchimeg, Badarch ;

Cao, Liang ;

Burger, Angelika M. ;

Ziche, Marina ;

Melillo, Giovanni .

CANCER RESEARCH, 2010, 70 (17) :6837-6848

[36] SYNTHESIS OF 4',5-OXYGENATED AND 3',4',5-OXYGENATED PYRANOISOFLAVONES - ALPINUMISOFLAVONE AND RELATED-COMPOUNDS, AND A REVISED STRUCTURE OF DERRONE [J].

TSUKAYAMA, M ;

KAWAMURA, Y ;

TAHARA, H .

HETEROCYCLES, 1992, 34 (03) :505-516

[37] Preparative isolation and purification of three rotenoids and one isoflavone from the seeds of Millettia pachycarpa Benth by high-speed counter-current chromatography [J].

Ye, Haoyu ;

Chen, Lijuan ;

Li, Yanfang ;

Peng, Aihua ;

Fu, Afu ;

Song, Hang ;

Tang, Minghai ;

Luo, Houding ;

Luo, Youfu ;

Xu, Yongbin ;

Shi, Jianyou ;

Wei, Yuquan .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1178 (1-2) :101-107

[38] Enrichment and isolation of barbigerone from Millettia pachycarpa Benth. using high-speed counter-current chromatography and preparative HPLC [J].

Ye, Haoyu ;

Zhong, Shijie ;

Li, Yanfang ;

Tang, Minghai ;

Peng, Aihua ;

Hu, Jia ;

Shi, Jie ;

He, Shicao ;

Wu, Wenshuang ;

Chen, Lijuan .

JOURNAL OF SEPARATION SCIENCE, 2010, 33 (08) :1010-1017

← 1 2 3 4 →