Allelic variation of HMW and LMW glutenin subunits, HMW secalin subunits and 75K gamma-secalins of hexaploid triticale

Although the endosperm storage protein of hexaploid triticale have already been analysed, the allelic diversity of glutenins and secalins remains to be described. Analysis, by SDS-PAGE, of about one thousand F2 seeds from ten triticale crosses allowed the inheritance of these storage proteins to be studied in order to determine their allelic forms and to assign them to particular chromosomes. Two new alleles encoding HMW subunits of glutenin and five new alleles encoding HMW subunits of secalin were determined at Glu-B1 and Glu-R1 loci respectively. In addition to the three allelic forms of 75K gamma-secalins encoded at Gli-R2 and previously reported, a null form was found. A nomenclature for these proteins and their corresponding alleles was suggested. The composition of B-LMW glutenin subunits of hexaploid triticale was described and allelic forms were determined: Five alleles were encoded at Glu-A3 and seven at Glu-B3 including one and two new allelic forms respectively.