In vitro cytotoxicity of laticifers extracts from Calotropis procera (Aiton) W.T and Pedilanthus tithymaloides (L.) Poit.

In order to assess the anticancer action of extracts obtained by latex from Calotropis procera and Pedilanthus tithymaloides, samples were collected from adult plants. Soluble proteins were extracted with 16 mu L of 50 mM sodium acetate pH 5/mu g integral latex and centrifugation at 16,000 x g for 15 min, the supernatant was named "latex crude extract" (LCE). The "latex methanolic extract" (LME) was obtained on dried latex. Both extracts were tested in vitro by cytotoxic and cytostatic activity in Jurkat cell cultures. Cellular viability, proliferation, necrosis and apoptosis were evaluated. LCE and LME of C. procera were found with cytotoxic and cytostatic activity after 24 incubation hours (p < 0,05) with doses from 1 mu g/mL. The LCE and LME of P. tithymaloides presented cytotoxic effect (p < 0,05) from 50 mu g/mL and from 1 mu g/mL, respectively.