Rhodanobacter koreensis sp nov., a bacterium isolated from tomato rhizosphere

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated THG-DD7(T), was isolated from tomato plant rhizosphere soil. Strain THG-DD7(T) grew optimally at 25-30 degrees C, at pH 7.0-7.5 and in the presence of 0.5% (w/v) NaCl. According to the results of 16S rRNA gene sequence comparisons, strain THG-DD7(T) was most closely related to Rhodanobacter umsongensis GR24-2(T) (98.2%), Rhodanobacter panaciterrae LnR5-47(T) (98.0%), Rhodanobacter soli DCY45(T) (97.9%), Rhodanobacter terrae GP18-1(T) (97.9%) and Dyella ginsengisoli Gsoil 3046(T) (97.7%). The DNA G+C content was 65.2 mol%. In DNA-DNA hybridization, the DNA relatedness levels between strain THG-DD7(T) and its closest phylogenetically neighbours were below 40.0%. The predominant isoprenoid quinone was ubiquinon Q-8. The major polar lipids were diphosphatidylglycerol, phosphtidylethanolamine, phosphatidyl-N-methylethanolamine and phosphatidylglycerol. The major fatty acids were iso-C-15:0, iso-C-16:0, isO-C-17:0, anteiso-C-15:0 and iSO-C-17:1 omega 9c and/or C-16:0 10-methyl (summed feature 9). These data supported the affiliation of strain THG-DD7(T) to the genus Rhodanobacter. The results of physiological and biochemical tests enabled strain THG-DD7(T) to be differentiated genotypically and phenotypically from the species of the genus Rhodanobacter with validly published names. Therefore, the novel isolate represents a novel species, for which the name Rhodanobacter koreensis sp. nov. is proposed. The type strain is THG-DD7(T) (=KACC 17650(T)=JCM 19614(T)).