A novel silica supported chitosan/glutaraldehyde as an efficient sorbent in solid phase extraction coupling with HPLC for the determination of Penicillin G from water and wastewater samples

In this study, silica@chitosan-glutaraldehyde (Si@Cs-G) was synthesized as a novel adsorbent for extraction of Penicillin G (PG) from the synthetic and real samples followed by HPLC determination. The synthesized adsorbents were characterized by the scanning electron microscopy (SEM), X-ray diffraction (XRD), fourier transform infrared (FTIR), dynamic light scattering (DLS), transmission electron microscopy (TEM) and nitrogen adsorption-desorption techniques. The factors influencing the extraction efficiency including pH, sorbent dose, extraction time, extraction solvent type and its volume were investigated and optimized. Under the optimal conditions (sorbent dosage: 25 mg, desorption solvent (acetonitrile) with volume of 0.75 mL; pH: 6 and extraction time: 50 min), the Si@Cs-G demonstrated high efficiency and linearity (R-2 > 0.999) with the concentration of penicillin G ranging from 1 to 300 mu g L-1. Extraction recovery in synthetic samples was 98.977%, with LOD = 0.493 mu g L-1, LOQ = 1.63 8 mu g L-1 and RSD < 1.953%. The method was successfully applied for determination of PG in real water samples (tap, river, lake and well water) and wastewater samples (SH and SHB hospital effluent). The obtained relative recoveries were in the range of 91.31%-123.27% with RSD less than 6.34% for all the real samples. The dominant mechanism in the PG adsorption process was involved in the pi-pi interaction, hydrogen bonding, and electrostatic interaction. (c) 2020 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).