Relaxation and Simplex mathematical algorithms applied to the study of steady-state electrochemical responses of immobilized enzyme biosensors: Comparison with experiments

被引：26

作者：

Flexer, V. ^{[2
]}

Pratt, K. F. E. ^{[3
]}

Garay, F. ^{[4
]}

Bartlett, P. N. ^{[1
]}

Calvo, E. J. ^{[2
]}

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Inorgan Analit & Quim Fis, INQUMAE, Buenos Aires, DF, Argentina

[3] City Technol Ltd, Portsmouth PO6 1SZ, Hants, England

[4] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Fisicoquim, INFIQC, RA-5000 Cordoba, Argentina

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2008年 / 616卷 / 1-2期

基金：

英国惠康基金;

关键词：

electrostatic self-assembly; enzyme electrodes; relaxation method; simplex algorithm;

D O I：

10.1016/j.jelechem.2008.01.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A description of the implementation of the relaxation method with automatic mesh point allocation for. immobilized enzyme electrodes is presented. The advantages of this method for the solution of coupled reaction-diffusion problems are discussed. The relaxation numerical simulation technique is combined with the Simplex fitting algorithm to extract kinetic parameters from experimental data. The results of the simulations are compared to experimental data from self-assembled multilayered electrodes comprised of glucose oxidase (GOx) and an Os modified redox mediator and found to be in excellent agreement. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：87 / 98

页数：12

共 37 条

[1] Redox enzymes immobilized on electrodes with solution cosubstrates. General procedure for simulation of time-resolved catalytic responses [J].