miR-TRAP: A Benchtop Chemical Biology Strategy to Identify microRNA Targets

被引：39

作者：

Baigude, Huricha ^{[1
]}

Ahsanullah ^{[1
]}

Li, Zhonghan ^{[1
]}

Zhou, Ying ^{[1
]}

Rana, Tariq M. ^{[1
]}

机构：

[1] Sanford Burnham Med Res Inst, Program RNA Biol, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 24期

基金：

美国国家卫生研究院;

关键词：

gene silencing; microRNA; mRNA; psoralen; noncoding RNAs; PREDICTION; BIOGENESIS; PSORALENS; PROTEIN; ELK-3; RNAS;

D O I：

10.1002/anie.201201512

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

It's a TRAP! microRNAs (miRNAs) are noncoding small RNAs that consist of approximately 21 nucleotides and are assembled into an RNA-induced silencing complex (RISC), which suppresses target mRNA expression. An easy-to-use method called miRNA target RNA affinity purification (miR-TRAP) directly identifies miRNA targets invivo. This method could be widely used to discover miRNA targets in various disease models under physiological conditions. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：5880 / 5883

页数：4

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