Continuous production of β-cyclodextrin from starch by highly stable cyclodextrin glycosyltransferase immobilized on chitosan

被引：53

作者：

Schoeffer, Jessie da Natividade ^{[1
]}

Klein, Manuela Poletto ^{[1
]}

Rodrigues, Rafael C. ^{[1
]}

Hertz, Plinho Francisco ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Inst Food Sci & Technol, Biotechnol Bioproc & Biocatalysis Grp, BR-91501970 Porto Alegre, RS, Brazil

来源：

CARBOHYDRATE POLYMERS | 2013年 / 98卷 / 02期

关键词：

Cyclodextrin glycosyltransferase; Enzyme immobilization; Packed bed reactor; Chitosan; ENZYME IMMOBILIZATION; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; CONTINUOUS BIOSYNTHESIS; REACTOR; CGTASE; CHITIN; CYCLODEXTRINGLYCOSYLTRANSFERASE; GLUCOSYLTRANSFERASE; THERMOANAEROBACTER; PERFORMANCE;

D O I：

10.1016/j.carbpol.2013.07.044

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacter sp. was covalently immobilized on glutaraldehyde-activated chitosan spheres and used in a packed bed reactor to investigate the continuous production of beta-cyclodextrin (beta-CD). The optimum temperatures were 75 C and 85 degrees C at pH 6.0, respectively for free and immobilized CGTase, and the optimum pH (5.0) was the same for both at 60 degrees C. In the reactor, the effects of flow rate and substrate concentration in the beta-CD production were evaluated. The optimum substrate concentration was 4% (w/v), maximizing the beta-CD production (1.32 g/L) in a flow rate of 3 mL/min. In addition, the biocatalyst had good operational stability at 60 C, maintaining 61% of its initial activity after 100 cycles of batch and 100% after 100h of continuous use. These results suggest the possibility of using this immobilized biocatalyst in continuous production of CDs. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：1311 / 1316

页数：6

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