Fetoplacental Oxygenation in an Intrauterine Growth Restriction Rat Model by Using Blood Oxygen Level-Dependent MR Imaging at 4.7 T

被引:36
作者
Chalouhi, Gihad E. [1 ,2 ]
Alison, Marianne [1 ,3 ]
Deloison, Benjamin [1 ,2 ]
Thiam, Rokhaya [1 ]
Autret, Gwennhael [1 ]
Balvay, Daniel [1 ]
Cuenod, Charles A. [1 ,4 ]
Clement, Olivier [1 ,4 ]
Salomon, Laurent J. [1 ,2 ]
Siauve, Nathalie [1 ,4 ]
机构
[1] Paris Cardiovasc Res Ctr PARCC, INSERM, U970, Paris, France
[2] Paris Descartes Univ, Hop Necker Enfants Malad, AP HP, Dept Obstet & Fetal Med, F-75015 Paris, France
[3] Paris Descartes Univ, Hop Robert Debre, AP HP, Dept Radiol, Paris, France
[4] Paris Descartes Univ, Hop Europeen Georges Pompidou, AP HP, Dept Radiol, Paris, France
关键词
WHITE-MATTER DAMAGE; MATERNAL HYPEROXYGENATION; FETAL; HYPOXIA; BRAIN; SATURATION; BOLD; RETARDATION; CONTRAST; T2-ASTERISK-MEASUREMENTS;
D O I
10.1148/radiol.13121742
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Purpose: To investigate blood oxygen level-dependent (BOLD) magnetic resonance (MR) imaging in an intrauterine growth restriction (IUGR) rat model as a noninvasive in vivo tool to evaluate the response of the fetoplacental units (FPUs) to oxygenation Materials and Methods: All procedures were approved by the animal care committee. The study was performed between February and July 2010. The IUGR model based on the ligation of the left uterine vascular pedicle at embryonic day 17 of gestation was validated by weighing placentas and fetuses after MR imaging. FPUs in the left and right uterine horns were IUGR cases and controls, respectively. A small-animal 4.7-T MR imager was used. Multiple gradient-echo sequence (repetition time msec/echo time msec, 800/1.8-49.8) was performed at embryonic day 19. T2* relaxation time was measured before and after maternal hyperoxygenation for live FPUs in placenta, fetal liver, and brain. The effect of hyperoxygenation on BOLD MR imaging was analyzed with change in T2* between hyperoxygenation and ambient air. After dissection, live fetuses from both horns were identified and weighed. Changes in T2* were compared based on Student t tests. A mixed model was used to compare BOLD effect among horns and organs. Results: Sixteen rats were studied. There was a significant fetal weight decrease in the IUGR FPUs (221.9%; P < .001). Change in T2* differed significantly between IUGR cases and controls for placenta (5.25 msec vs 11.25 msec; P < .001) and fetal brain (3.7 msec vs 7.17 msec; P = .02), whereas there was no significant difference in the fetal liver (2.72 msec vs 3.18 msec; P = .47). Conclusion: BOLD MR imaging at 4.7 T can be used to evaluate the response to oxygenation in normal and IUGR FPUs. This technique has a potential role in the assessment of human pregnancy. (C) RSNA, 2013
引用
收藏
页码:122 / 129
页数:8
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